Apply CRISPR-Cas9 gene editing to high-throughput screening

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The CRISPR-Cas9 gene editing system provides an efficient approach for specific, complete, and permanent knockout of gene expression, making it a potent research tool for determining key players in specific biological pathways. The new Invitrogen™ LentiArray™ CRISPR libraries extend CRISPR-Cas9 technology into high-throughput applications for functional genomic screening. LentiArray libraries enable the interrogation of hundreds or thousands of genes in a single experiment with:

  • Advanced guide RNA (gRNA) designs for maximum knockout efficiency without sacrificing specificity
  • Up to 4 high-quality gRNAs per gene target, for efficient knockout in a wide variety of cell types
  • Two choices for delivery—high-titer, ready-to- use lentivirus, or glycerol stocks of E. coli containing lentiviral plasmids
  • A complete set of controls and lentiviruses against single-gene targets to support pre-screen assay development and rapid post-screen hit validation
  • 19 defined libraries and custom options available, enabling screens of defined gene sets or unbiased surveys of the whole genome (Table 1)

LentiArray library specifics

For example, the LentiArray Human Whole Genome CRISPR Library targets 18,453 genes with up to 4 gRNAs per gene target (pooled in a single well), for a total of 73,812 gRNAs. The gene targets within this library were selected using the most up-to-date genome databases, including the NCBI RefSeq database, and cross-referenced to the Gene Ontology Consortium (GO) database or the HUGO Gene Nomenclature Committee (HGNC). LentiArray CRISPR libraries are constructed using our proprietary CRISPR gRNA design algorithm, which incorporates the latest gRNA design research; gRNAs are selected for maximal editing efficiency and specificity and are designed to knock out all known isoforms of the target gene. Libraries are delivered as 100 μL of ready-to-use lentiviral particles per gene target at a titer of 1 x 108 TU/mL (functional titer determined by antibiotic resistance) and are also available as glycerol stocks (Table 1).

Table 1. Available defined libraries, supporting focused high-throughput screens as well as unbiased whole genome surveys.

Product No. of genes Cat. No.
(ready to use)
Cat. No.
(glycerol stocks)
LentiArray Human Whole Genome CRISPR Library 18,453 Inquire A32185
LentiArray Human Druggable Genome CRISPR Library 10,128 Inquire A32184
LentiArray Human Apoptosis CRISPR Library 904 A42275 A32176
LentiArray Human Cancer Biology CRISPR Library 510 A42268 A32169
LentiArray Human Cell Cycle CRISPR Library 1,444 A42271 A32172
LentiArray Human Cell Surface CRISPR Library 778 A42278 A32179
LentiArray Human DNA Damage Response CRISPR Library 561 A42281 A32182
LentiArray Human Drug Transport CRISPR Library 98 A42276 A32177
LentiArray Human Epigenetics CRISPR Library 396 A42269 A32170
LentiArray Human GPCR CRISPR Library 446 A42282 A32183
LentiArray Human Ion Channel CRISPR Library 328 A42277 A32178
LentiArray Human Kinase CRISPR Library 840 A42234 A32167
LentiArray Human Membrane Trafficking CRISPR Library 141 A42272 A32173
LentiArray Human Nuclear Hormone Receptor CRISPR Library 47 A42274 A32175
LentiArray Human Phosphatase CRISPR Library 288 A42267 A32168
LentiArray Human Protease CRISPR Library 475 A42279 A32180
LentiArray Human Transcription Factor CRISPR Library 1,817 A42273 A32174
LentiArray Human Tumor Suppressor CRISPR Library 716 A42280 A32181
LentiArray Human Ubiquitin CRISPR Library 943 A42270 A32171

Learn more about the LentiArray libraries

LentiArray CRISPR libraries are delivered in an arrayed format compatible with existing high-throughput screening infrastructure and have been designed and constructed to provide a flexible system that doesn’t impose limitations on your assay design. They utilize a two-vector design, expressing the Cas9 nuclease and the gRNA from separate lentiviral constructs, enabling you to dictate when and how the genome editing tools are delivered to your cells. Explore the LentiArray CRISPR libraries and find out how CRISPR-Cas9 technology can expand your screening capabilities.

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