Amplify signals for fluorescence imaging: SuperBoost Kits with Alexa Fluor tyramides for signal amplification
Many functionally important proteins such as transcription factors and cell-surface cytokine receptors have native expression levels far below the detection threshold of labeled primary and secondary antibodies. The Invitrogen SuperBoost kits with Alexa Fluor tyramides provide a highly sensitive method for detecting low-abundance targets in immunocytochemistry (ICC), immunohistochemistry (IHC), and in situ hybridization (ISH) applications. Tyramide SuperBoost technology combines the brightness of Alexa Fluor dyes with poly-HRP–mediated tyramide signal amplification to discern signal from noise, yielding precision and sensitivity 10–200 times greater than that of standard ICC/IHC/ISH methods and 2–10 times greater than that of other tyramide amplification techniques, including traditional TSA™ methods.
Fluorophore and Reagent Selection Guide for Flow Cytometry
The Fluorophore and Reagent Selection Guide poster in the center of BioProbes 75 highlights the recommended fluorescent dyes and fluorescent proteins for six different excitation lasers, as well as their common emission filters and Attune NxT channel. Also included for each laser are dyes for viability, DNA content/cell cycle, cell proliferation, apoptosis, reactive oxygen species, and phagocytosis assays. Use this poster to choose the best reagents for your next flow cytometry experiment.
Tools for fluorescence imaging
Amplify signals for fluorescence imaging
SuperBoost kits with Alexa Fluor tyramides for signal amplification
Cancer biology in the third dimension
Fluorescent reagents for staining 3D cell cultures created with Cell-Mate3D matrix
Research with antibodies
Harness immune checkpoints to combat tumors
Immune checkpoint antibodies for flow cytometry, IHC, and functional bioassays
Comprehensive strategy for antibody validation
Characterization of antibody performance using immunoprecipitation and mass spectrometry
Examine signaling pathways with targeted proteomics
Quantitative analysis of the AKT/mTOR pathway using multiplex immunoprecipitation and targeted mass spectrometry
Tools for flow cytometry
Bright fluorescent polymer dyes for the violet laser
Super Bright antibody conjugates
Powering up drug discovery
Cancer biology applications using the Attune NxT Flow Cytometer
Evaluate both RNA and protein targets in single cells
PrimeFlow RNA assay for detecting RNA targets by flow cytometry
Strengthening our flow cytometry portfolio: eBioscience antibodies
At Thermo Fisher Scientific, we are committed to furthering your scientific advances by providing a comprehensive suite of products for the analysis of cells and their functions. The addition of the eBioscience portfolio of flow cytometry antibodies and reagents to the innovative Invitrogen flow cytometry reagents and instruments enables us to better provide the tools your experiments demand. We are fully committed to maintaining product quality while also making the transition as seamless as possible for you. Beyond our products, we also provide dedicated service and support teams and educational resources to help support your studies. From antibodies and functional reagents to instrumentation, Thermo Fisher Scientific is now poised to more fully serve your research needs. We are focused on advancing meaningful discoveries and partnering to make tools for cellular analysis effective, affordable, and widely accessible for all life scientists.
Learn more about our wide-ranging array of flow cytometry products at thermofisher.com/flowcytometry
Behind the Bench blog goes with the flow
Do you work with a flow cytometer? Do you want to learn more from experienced scientists in the field? Thermo Fisher Scientific now has an archive of flow cytometry articles available on the “Behind the Bench” blog. Learn about “Flow cytometer fluidics and why they are not all the same” from Greg Kaduchak, PhD, Thermo Fisher Scientific R&D Engineer. Studying cell proliferation using CFSE or BrdU? We have the blog for you! Get advice from Andrew Filby, PhD, Flow Cytometry Core Facility Director and ISAC SRL-Emerging Leader at Newcastle University, UK, or Bill Telford, PhD, Flow Cytometry Research Core Manager at the National Cancer Institute, NIH, Bethesda, Maryland, to help you get the most from your CFSE- or BrdUbased cell proliferation assays.
Read all about it at thermofisher.com/flowblog
eLearning modules for protein and cell analysis
We are pleased to announce the release of several eLearning modules for protein and cell analysis applications. Available in the new and improved Protein and Cell Analysis Education virtual environment, these self-paced animated courses include knowledge checks throughout and a practical applications component to help you see what you have learned. The first courses cover:
- T cell stimulation and proliferation: Part 1 and Part 2
- Protein sample preparation—Techniques and applications: Part 1 and Part 2
All content is available 24 hours a day, 7 days a week, and is viewable from the convenience of your desk, tablet, or mobile device.
See what it's all about at thermofisher.com/elearningcourses
The newest Molecular Probes School of Fluorescence module: Fluorescence Basics
The module “Fluorescence Basics” is now available online at the Molecular Probes School of Fluorescence. It covers the fundamentals of fluorescence, from the process of fluorescence and photobleaching to the anatomy of a fluorescence spectrum and how filters work, and is brought to you by the scientists who created the Molecular Probes Handbook and the “Imaging Basics” module.
Check it out today at thermofisher.com/mpsf-fluorescence
Antibodies Learning Center now online
Invitrogen primary and secondary antibodies represent a large and ever-improving portfolio of products designed to help researchers identify, locate, quantitate, and purify proteins and other biomolecules. Selecting individual antibody products from among hundreds of options can be a challenge. The Thermo Fisher Scientific Antibodies Learning Center contains educational materials designed to empower researchers and technicians with the background information about immunotechnologies that is necessary to develop, select, and use antibodies to advance their research.
Learn more at thermofisher.com/ablearning
Protein purification tools poster available upon request
Now available upon request, the protein purification tools poster provides helpful information about the Thermo Scientific and Invitrogen portfolios of magnetic beads and magnetic agarose, as well as Superflow and POROS resins for applications ranging from small-scale high-throughput screening to process-scale purification. This poster features practical information, including binding capacity, maximum flow rates, and the recommended application scale and packaging options for each product. Key upstream and downstream products are also highlighted as part of the integrated protein purification workflows.
Request your free poster today (see terms and conditions) at thermofisher.com/purificationposter
Mass spectrometry technical handbook now available
The Mass Spectrometry Resources Center provides valuable tools to help with your mass spectrometry experiments, including a free 166-page technical handbook, Protein sample preparation and quantitation for mass spectrometry. In addition, you’ll have access to helpful white papers and late-breaking posters for specific applications like subcellular fractionation, crosslinking, peptide fractionation, isobaric labeling, and more. You can also hear from our experts on sample preparation and targeted proteomics workflows through our on-demand webinars.
Register now at thermofisher.com/msresources
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
For Research Use Only. Not for use in diagnostic procedures.