Introducing Zip Alexa Fluor Rapid Antibody Labeling Kits

The Invitrogen Zip Alexa Fluor Rapid Antibody Labeling Kits provide reagents and a simple protocol to efficiently label your primary antibody with one of three bright fluorescent Alexa Fluor dyes in just 15 minutes. Not only is the antibody conjugate ready to use in this short time, but you will recover 100% of your antibody because there are no purification steps required. Using a directly labeled primary antibody often produces lower background fluorescence and less nonspecific binding than detection with a secondary antibody. In addition, multiple primary antibodies of the same isotype or derived from the same species can easily be used in the same experiment if they are directly labeled with compatible fluorophores.

The kit contains everything you need to perform three separate labeling reactions and produces fluorescent antibody conjugates that are ideal for a wide range of applications, including flow cytometry, fluorescent microscopy, immunohistochemistry, immunocytochemistry, ELISAs, and indirect FISH.

Learn more about our diverse selection of antibody and protein labeling kits

Antibody-labeled HeLa cells

HeLa cells stained with directly labeled primary antibodies. HeLa cells were labeled with a mouse anti-Ki67 antibody directly conjugated to Alexa Fluor 488 dye using the Invitrogen Zip Alexa Fluor 488 Rapid Antibody Labeling Kit and with Alexa Fluor Plus 405 Phalloidin. Cells were imaged using a 40x objective on the Thermo Scientific CellInsight CX7 LZR High-Content Analysis Platform using spinning disk confocal microscopy.


Next-generation ProQuantum high-sensitivity immunoassays

Meet the newest easy-to-run immunoassay kits for measuring low levels of cytokine proteins using very small (2 μL) sample volumes. Leveraging a proximity-based amplification technology, the Invitrogen ProQuantum immunoassays combine the analyte specificity of high-affinity antibody–antigen binding with the high sensitivity and large dynamic range of real-time PCR amplification to achieve simple yet powerful protein quantitation. ProQuantum immunoassays are an open, affordable platform that can be run on any qPCR instrument.

Find out more about the ProQuantum high-sensitivity immunoassays

How ProQuantum immunoassays work

How ProQuantum immunoassays work. Paired antibody–oligonucleotide conjugates bind to the analyte of interest during a 1 hr incubation, followed by ligation of the two oligonucleotides in the presence of a splint oligonucleotide and 40 cycles of qPCR-based amplification. No wash steps are required, and the amount of analyte-specific antibody pair binding is directly proportional to the number of PCR amplicons generated.


Improved tools for labeling the actin cytoskeleton

Fluorescent phallotoxin derivatives, which stain F-actin selectively at nanomolar concentrations, are powerful labels for identifying and quantifying actin in fixed cells, tissue sections, or cell-free preparations. Thermo Fisher Scientific offers over 26 different fluorescent phalloidins conjugated either to Invitrogen Alexa Fluor dyes or to classic fluorophores. With recent protocol improvements for making stock solutions, our phalloidin products now produce up to 60% more signal than previous formulations, with an improved workflow.

In addition, we have just added two very bright fluorescent phalloidin conjugates to the portfolio, increasing your labeling choices and facilitating 4- to 6-plex imaging without interference from spectral overlap. The near-infrared–excitable Alexa Fluor Plus 750 Phalloidin can be detected with the 790 nm laser line of the Thermo Scientific CellInsight CX7 LZE High-Content Analysis Platform or other confocal microscope, the Invitrogen EVOS Cy7 Light Cube, or a Cy7 filter set. The violet light–excitable Alexa Fluor Plus 405 Phalloidin can be detected with the 405 nm laser line, the EVOS DAPI Light Cube, or a DAPI filter set.

Find out more about our complete set of actin-specific probes

HeLa cells labeled with Alexa Fluor Plus 750 Phalloidin

HeLa cells labeled with Alexa Fluor Plus 750 Phalloidin. HeLa cells were labeled overnight with Invitrogen CellLight Talin-GFP (BacMam 2.0), CellLight Mitochondria-RFP (BacMam 2.0), and NucBlue Live ReadyProbes Reagent, and then fixed, permeabilized, and labeled with anti–α-tubulin mouse monoclonal antibody (clone DM1A), in conjunction with Alexa Fluor 647 goat anti–mouse IgG secondary antibody and Alexa Fluor Plus 750 Phalloidin. Cells were mounted in ProLong Glass Antifade Mountant. Images were acquired using an EVOS FL Auto 2 Imaging System with an Olympus 60x Aprochromat Oil Objective and EVOS DAPI, GFP, RFP, and Cy7 light cubes.


Tubulin Tracker Deep Red for labeling polymerized tubulin

The Invitrogen Tubulin Tracker reagents are cell-permeant fluorescent dyes that specifically label polymerized tubulin in live cells. Tubulin Tracker Deep Red—a conjugate of docetaxel and a very bright and photostable far-red fluorophore—easily enters live cells and stains tubulin with deep red fluorescence. Tubulin Tracker Deep Red (excitation/emission = 652/669 nm) can be visualized with standard Cy5 filter settings using almost any fluorescence imaging instrument. This far-red–fluorescent tubulin probe can be multiplexed with blue, green, orange, red, and near-IR fluorophores. Both Tubulin Tracker Deep Red and the original Tubulin Tracker Green are fluorescent derivatives of cytoskeletal drugs (docetaxel and paclitaxel, respectively) that promote and preserve tubulin polymerization. To learn more, visit thermofisher.com/tubulintracker.

Learn more about Tubulin Tracker probes
Featured scientific poster: Visualizing microtubule dynamics in live cells

Neurons stained with Tubulin Tracker Deep Red

Rat cortical neurons stained with Tubulin Tracker Deep Red. Gibco Primary Rat Cortex Neurons were stained with Invitrogen Tubulin Tracker Deep Red and imaged in Invitrogen Live Cell Imaging Solution on an Invitrogen EVOS FL Auto 2 Imaging System with a 20x objective and an Invitrogen EVOS Cy5 Light Cube.


Rapid sample prep for mass spectrometry analysis

Traditional protein sample preparation for mass spectrometry (MS) analysis requires numerous and variable steps and can take up to 2 days to complete. The Thermo Scientific EasyPep Mini MS Sample Prep Kit provides preformulated reagents, an MS-grade enzyme mix, peptide clean-up columns, and a robust method for the preparation of high-quality samples for MS analysis in just 3 to 5 hours. This kit enables efficient and reproducible processing of cultured mammalian cells, plasma, and tissue (10–100 μg), providing more protein identifications and higher peptide yields with fewer steps than traditional methods.

Learn more about the EasyPep Mini MS Sample Prep Kit

EasyPep Mini MS Sample Prep workflow

Comparison of the traditional workflow for mass spectrometry sample prepara­tion and the EasyPep Mini MS Sample Prep workflow.


Flow cytometry antibodies: More markers, more clones, more formats

As a committed supplier of flow cytometry antibodies, Thermo Fisher Scientific has already released more than 1,300 flow cytometry antibody conjugates in 2018, providing you with more options when designing flow cytometry panels. Highlights of recently released markers include:

  • Anti–mouse CD294 (CRTH2) antibody—a unique monoclonal for studying innate immunity, for use with eosinophils and mast cells
  • Anti–human and anti–mouse VISTA (B7-H5, PD-1H) antibodies—for studying hematopoietic cells in immuno-oncology applications
  • Anti–arginase 1 antibody (clone A1exF5)—the only clone available that can detect human or mouse arginase 1 by flow cytometry

Additionally, we’ve continued to support and extend our portfolio of violet light–excitable Invitrogen eBioscience Super Bright antibody conjugates, and we now offer Invitrogen eBioscience Super Bright Staining Buffer in two convenient sizes.

Search the entire portfolio of 13,000 flow cytometry antibodies

Expression of CD294

Expression of CD294 (CRTH2) in lysed whole blood cells from mice. Swiss Webster mouse lysed whole blood cells were stained with Invitrogen CD170 (Siglec F) Monoclonal Antibody (clone 1RNM44N), PerCP-eFluor 710, eBioscience and either 0.125 µg of Invitrogen Rat IgG2a kappa Isotype Control, Alexa Fluor 647, eBioscience (left) or 0.125 µg of Invitrogen CD294 (CRTH2) Monoclonal Antibody (clone No3m1scz), Alexa Fluor 647, eBioscience (right). Total viable cells were used for this flow cytometry analysis.


Pierce Chromogenic Endotoxin Quant Kit with improved sensitivity and broad-range detection

Recognizing the need for a more sensitive endotoxin detection method, Thermo Fisher Scientific has extended its endotoxin detection line to include the Thermo Scientific Pierce Chromogenic Endotoxin Quant Kit, which accurately detects endotoxins at levels as low as 0.01 EU/mL in samples. This kit is an endpoint amebocyte lysate assay that quantifies endotoxins (lipopolysaccharides) in a variety of sample types, including protein, vaccine, plasmid, DNA, and RNA samples, with no interference from β-glucans. The kit offers high sensitivity with two linear dynamic ranges of 0.01–0.1 and 0.1–1.0 EU/mL, and reproducibility from test to test and operator to operator with a coefficient of variation (CV) of 3%.

Find out more about endotoxin quantitation

Pierce Chromogenic Endotoxin Quant Kit

Comparison of Pierce endotoxin quantitation kits. Compared with the Thermo Scientific Pierce LAL Chromogenic Endotoxin Quantitation Kit, the Thermo Scientific Pierce Chromogenic Endotoxin Quant Kit provides a nearly identical linear response curve but with an increased dynamic range of 0.01–0.1 EU/mL. Assays were performed according to the manufacturer’s protocols. The standard curves for both kits produced R2 ≥ 0.98, and a CV <5%.


Quantitative mass spectrometry–based targeted assays for cancer signaling pathways

Thermo Scientific SureQuant Panels enable multiplex immunoprecipitation to mass spectrometry (mIP-MS) analysis for simultaneous enrichment and quantitation of multiple total and phosphorylated proteins in the AKT/mTOR signaling pathway. Each multiplex panel for absolute or relative quantitation contains two modules: 1) the IP and MS Sample Prep Module, which includes all reagents necessary to immunoenrich AKT/mTOR pathway proteins and perform in-solution MS sample preparation; and 2) the Absolute or Relative Quantitation Module, which includes a system suitability standard and AQUA Ultimate Heavy and Light Peptide (or, in the Relative Quantitation module, only Heavy Peptide) mixtures. The immunoenriched and digested samples spiked with internal standards can be processed using the discovery MS method (nanoLC-MS/MS) or targeted MS method (nanoLC-PRM/ MS).

See our full portfolio of mass spectrometry kits for cancer signaling pathways

SureQuant AKT Pathway (Phospho) Multiplex Panel

Absolute quantitation of AKT (phospho) signaling pathway proteins in mammalian cells using the SureQuant AKT Pathway (Phospho) Multiplex Panel (Absolute Quantitation). Absolute quantitation of AKT/mTOR phospho pathway proteins was performed using the mammalian cell line MCF7 treated with IGF1. AKT/mTOR pathway proteins were enriched through multiplex immunoprecipitation, followed by nanoLC-PRM/MS analyses on a Thermo Scientific Q Exactive HF-Orbitrap Mass Spectrometer. All targets were quantified by standard curves generated for each target peptide included in the Quantitation Modules.

Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.