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Adoptive cell therapies in immuno-oncology research

Recent advances in immuno-oncology (I-O) research are paving the way for the discovery of novel classes of cancer therapeutics that enhance or enable antitumor immune responses, overcome tumor evasion mechanisms, and promote conditions that favor immune protection. Two principal areas of research include the development of biotherapeutic antibodies that target immune-checkpoint pathways and the expansion of cellular approaches such as adoptive cell therapies (ACT). Thermo Fisher Scientific is developing 3D cell culture techniques and low–cell attachment microplates, along with assays and instruments for analyzing cell function. Here we demonstrate the use of several of these techniques and reagents to study immune cell infiltration in 3D tumor spheroid cultures and the subsequent death, by apoptosis, of spheroid cells.

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About the cover

First place winner of the Thermo Fisher Scientific Cell-ebrate Science Imaging Contest in Singapore, Taiwan, and the Southeast Asia region. A human skin cancer section was probed with antibodies for keratin 14 and diaphanous 1, followed by detection with Invitrogen Anti–Mouse IgG Secondary Antibody, Alexa Fluor 488 (for keratin 14) and Invitrogen Anti–Rabbit IgG Secondary Antibody, Alexa Fluor 568 (for diaphanous 1). The section was counterstained with DAPI nucleic acid stain. The image was acquired using an Olympus™ FLUOVIEW™ FV1000 confocal laser-scanning microscope. Image contributed by Mohsin Bin Bashir, A*STAR Institute of Medical Biology (IMB), Singapore.

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Immuno-oncology research

Adoptive cell therapies in immuno-oncology research
Analyze immune cell cytotoxicity in 3D tumor models

Tools for flow cytometry

Best practices for multiparameter flow cytometry
Experimental setup and panel design for accurate results

Detect bacteria in both research and industrial samples
New applications for flow cytometric analysis

Cell function assays

Fluorescent detection of senescence for imaging and flow cytometry applications
Multiplex with the CellEvent Senescence Green Probe for a more complete picture

Monitor drug-induced calcium flux in iPSC-derived cardiomyocytes
Fluo-4 meets the EVOS FL Auto 2 Imaging System and Celleste Image Analysis Software

Visualize microtubule dynamics in live cells
With the highly photostable Tubulin Tracker Deep Red reagent

Assess the cell viability of Staphylococcus aureus biofilms
PrestoBlue HS and alamarBlue HS reagents for microplate viability assays

Antibodies and immunoassays for pathway research

SNAP-ChIP: A robust method for determining histone antibody specificity in ChIP
Barcoded synthetic nucleosomes serve as ChIP internal controls

Hunting for Hippo proteins
With highly specific ABfinity recombinant monoclonal antibodies

Role of metabolic pathways in cancer
Using siRNA knockdowns to verify antibody specificity

Dissect signaling pathways with multiplex western blots
Using fluorescent antibody conjugates in combination with the iBright Imaging System

ELISA workflows just got easier
With Instant ELISA kits, E1-ClipTip electronic pipette, and Wellwash Versa Microplate Washer

Measure secreted protein and mRNA levels with a single instrument platform
ProcartaPlex and ProQuantum immunoassays augment mRNA quantitation

Journal club

Therapeutic targeting of sunitinib-induced androgen receptor (AR) phosphorylation in renal cell carcinoma

Across the spectrum: Supporting spectral flow cytometry applications

Spectral flow cytometry exploits the inherent emission pattern of each fluorescent molecule to generate a unique spectral signature. By relying on the discrimination of unique spectral signatures rather than specific emission channels for detection, spectral flow cytometry enables the use of many fluorescent combinations that were previously difficult or impossible to separate, allowing for increasingly complex multicolor experiments.

The Invitrogen portfolio of flow cytometry antibodies, assays, and reagents addresses the needs of your spectral flow cytometry experiment, whether you are an immunologist investigating the role of regulatory T cells (Tregs), a cell biologist interested in fluorescently labeling RNA for downstream applications, or a microbiologist studying endocytosis, viability, or proliferation.

Learn more about spectral flow cytometry assays and reagents

Behind the Bench blog: Macrophages and more macrophages

Want to learn a few basics about macrophages? Interested in hearing about the newest antibody markers available for studying macrophages? The Invitrogen Behind the Bench blog has four new blog posts that cater to your macrophage research.

Get macrophage basics directly from a Thermo Fisher Scientific R&D scientist in the blog post “Macrophages by flow cytometry: New insights through novel antibody reagents”. Or, for a description of available antibody markers for studying macrophages, read three new blog posts from the R&D scientists who developed them:

Each blog post provides insights into the performance of the antibody markers and their applications in the immunophenotyping of macrophages using flow cytometry.

Read the flow cytometry–tagged subset of the Behind the Bench blog

Watch: How to use the Pierce Chromogenic Endotoxin Quantitation Kit

The video “How to use the Pierce Chromogenic Endotoxin Quantitation Kit” brings to life the simple protocol of the Thermo Scientific Pierce Chromogenic Endotoxin Quant Kit. In just over 10 minutes, you will learn how this kit accurately detects and measures endotoxin (lipopolysaccharide) in a protein, peptide, nucleic acid, or antibody sample using an endpoint amebocyte lysate assay. The Pierce Chromogenic Endotoxin Quant Kit enables endotoxin contamination detection with two linear sensitivity ranges: 0.01–0.1 EU/mL and 0.1–1.0 EU/mL. It is also:

  • Highly specific—no interference from β-glucans and suitable for a wide range of samples
  • Fast—perform assay in as little as 20 minutes
  • Chromogenic—measure this endpoint assay using a standard spectrophotometer or plate reader at 405–410 nm

Watch the video How to use the Pierce Chromogenic Endotoxin Quantitation Kit