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3D midbrain organoid model development

The drug development path—from hit discovery to the clinical space—relies on accurate experimental data that are predictive of outcomes in downstream settings. Increasing the complexity of disease models with 3D organoids in the early stages of drug discovery is an approach that provides a more relevant cellular context and potentially more accurate physiological data in orthogonal experiments. The development of 3D organoids that better represent the corresponding organ complexity enables researchers to better scale experiments, make data-driven decisions, and systemize those processes to speed discovery.

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Fluorescence imaging of a labeled macrophage. Monocytes were isolated from human peripheral blood mononuclear cells (PBMCs) using Invitrogen Dynabeads Untouched Human Monocytes Kit, then differentiated into macrophages for 7 days in Gibco RPMI 1640 Medium with 10% Gibco Fetal Bovine Serum, 8% human serum, 1% Gibco GlutaMAX Supplement, 20 mM HEPES, 1% Gibco Penicillin-Streptomycin (10,000 U/mL), and 50 ng/mL Gibco M-CSF Recombinant Human Protein. Macrophages prepared in this manner are useful models for studying antibody-dependent phagocytosis of cancer cells or phagocytosis of microorganisms. These human monocyte–derived macrophages were cultured in a 96-well microplate before labeling with Invitrogen CellTrace Violet dye (pseudocolored red), which labels the entire cell but the cell body most intensely, and a fluorescent live-cell actin tracking stain (pseudocolored purple). This labeled macrophage (~50 μm in diameter) was imaged using the Invitrogen EVOS M7000 Imaging System with an Olympus 60x apochromat oil objective.


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Characterizing functional immuno-oncology
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Immunodetection of proteins in the innate immune system
Antibodies for nucleic acid sensing pathways

Phenotyping on the front lines
Flow cytometry antibodies for identifying tissue-resident memory T cells

Assay optimization for 3D cell cultures

Assess cell health and function in 2D and 3D cell cultures
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Benchtop nucleic acid quantitation

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Accurate and precise quantitation of up to 8 samples simultaneously

Journal club

The effects of inflammation on the mutational spectrum of clonal hematopoiesis of indeterminate potential (CHIP)


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On-demand webinar: Strategies for isolation of plasma membrane proteins

Isolating plasma membrane proteins presents many challenges, and it can often prove difficult to choose the right method based on the sample and downstream application. This webinar focuses on robust and optimized techniques for extraction, isolation, and enrichment of cell-surface proteins, including stable and functional G protein–coupled receptors (GPCRs). In this webinar, we present:

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Updated: The Attune NxT Flow Cytometer reference article database

Scientists at Thermo Fisher Scientific have established a reference article database that features over 475 peer-reviewed publications citing the use of the Invitrogen Attune NxT Flow Cytometer. You can easily perform keyword searches (e.g., author, cell type, species) to access hundreds of articles in various areas of life science research, from immunology, immunotherapy, cancer, and drug discovery, to food, agriculture, and veterinary and materials sciences. It includes scientific articles published through 2019, and we are committed to updating it with new references as they are published. Learn more about how the Attune NxT Flow Cytometer can enhance your research with its ability to provide consistent results, whether samples are dilute or concentrated, require fast or slow flow rates, or are susceptible to clogging during analysis.

Explore the Attune NxT reference database

Behind the Bench blog: The latest antibody news

We are continually updating our Invitrogen antibody portfolio with validated* primary antibodies for use in flow cytometry, IF/ICC/IHC, western blotting, and ELISAs. Check out these recent blog posts, showcasing the latest antibody data for selected nucleic acid and protein research topics:

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  • Drivers of the chromosomal passenger complex
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*The use or any variation of the word “validation” refers only to research use antibodies that were subject to functional testing to confirm that the antibody can be used with the research techniques indicated. It does not ensure that the product(s) was validated for clinical or diagnostic uses.

Best practices: 5 steps to intracellular flow cytometry

The ability to detect intracellular proteins with flow cytometry opens the door to a more complete characterization of cell populations and distinct subsets within those populations. To help you save time and effort and increase your efficiency when designing flow cytometry experiments, we have organized our resources, tools, and products for intracellular flow cytometry into 5 simple steps:

  • Step 1: Target determination, with a link to our antibody search tool
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