The drug development path—from hit discovery to the clinical space—relies on accurate experimental data that are predictive of outcomes in downstream settings. Increasing the complexity of disease models with 3D organoids in the early stages of drug discovery is an approach that provides a more relevant cellular context and potentially more accurate physiological data in orthogonal experiments. The development of 3D organoids that better represent the corresponding organ complexity enables researchers to better scale experiments, make data-driven decisions, and systemize those processes to speed discovery.
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Fluorescence imaging of a labeled macrophage. Monocytes were isolated from human peripheral blood mononuclear cells (PBMCs) using Invitrogen Dynabeads Untouched Human Monocytes Kit, then differentiated into macrophages for 7 days in Gibco RPMI 1640 Medium with 10% Gibco Fetal Bovine Serum, 8% human serum, 1% Gibco GlutaMAX Supplement, 20 mM HEPES, 1% Gibco Penicillin-Streptomycin (10,000 U/mL), and 50 ng/mL Gibco M-CSF Recombinant Human Protein. Macrophages prepared in this manner are useful models for studying antibody-dependent phagocytosis of cancer cells or phagocytosis of microorganisms. These human monocyte–derived macrophages were cultured in a 96-well microplate before labeling with Invitrogen CellTrace Violet dye (pseudocolored red), which labels the entire cell but the cell body most intensely, and a fluorescent live-cell actin tracking stain (pseudocolored purple). This labeled macrophage (~50 μm in diameter) was imaged using the Invitrogen EVOS M7000 Imaging System with an Olympus 60x apochromat oil objective.
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3D midbrain model using neural organoids
3D midbrain organoid model development
Floor plate differentiation of iPSC-derived dopaminergic neurons in 3D culture
Antibodies and immunoassays
Characterizing functional immuno-oncology
Using flow cytometry and high-content imaging to evaluate innate immune function
Immunodetection of proteins in the innate immune system
Antibodies for nucleic acid sensing pathways
Phenotyping on the front lines
Flow cytometry antibodies for identifying tissue-resident memory T cells
Assay optimization for 3D cell cultures
Assess cell health and function in 2D and 3D cell cultures
Optimizing microplate assay protocols for spheroid cultures
Benchtop nucleic acid quantitation
Introducing the new Qubit Flex Fluorometer: DNA, RNA, and protein quantitation with flexible throughput
Accurate and precise quantitation of up to 8 samples simultaneously
More choices with new antibodies from selected suppliers
Target-specific antibodies are critical reagents in today’s cutting-edge research, driving the need for more choices. We are partnering with selected antibody vendors to provide you online access to more primary antibodies from multiple companies.
To easily find what you need, utilize the enhanced online search tool to search, compare, and purchase antibodies from our expansive portfolio. Start by typing in the protein target to find the most relevant options, and then use filters to narrow the search. You can explore relevant and detailed information such as published figures, advanced verification data, references, images, and citations. Compare these key product features to help you make your decision. Additional useful tools include the ability to create and share lists with colleagues or request custom or bulk orders.
Get started today with our antibody selection tool
On-demand webinar: Strategies for isolation of plasma membrane proteins
Isolating plasma membrane proteins presents many challenges, and it can often prove difficult to choose the right method based on the sample and downstream application. This webinar focuses on robust and optimized techniques for extraction, isolation, and enrichment of cell-surface proteins, including stable and functional G protein–coupled receptors (GPCRs). In this webinar, we present:
- Techniques for isolating plasma membrane proteins
- Ways to produce stable and functional GPCR extracts
- Enrichment methods for cell-surface proteins that produce minimal off-target contaminants
- How cell-surface proteins can serve as a treasure trove of pharma-relevant targets for potential drug therapy
View this on-demand webinar
Updated: The Attune NxT Flow Cytometer reference article database
Scientists at Thermo Fisher Scientific have established a reference article database that features over 475 peer-reviewed publications citing the use of the Invitrogen Attune NxT Flow Cytometer. You can easily perform keyword searches (e.g., author, cell type, species) to access hundreds of articles in various areas of life science research, from immunology, immunotherapy, cancer, and drug discovery, to food, agriculture, and veterinary and materials sciences. It includes scientific articles published through 2019, and we are committed to updating it with new references as they are published. Learn more about how the Attune NxT Flow Cytometer can enhance your research with its ability to provide consistent results, whether samples are dilute or concentrated, require fast or slow flow rates, or are susceptible to clogging during analysis.
Explore the Attune NxT reference database
Behind the Bench blog: The latest antibody news
We are continually updating our Invitrogen antibody portfolio with validated* primary antibodies for use in flow cytometry, IF/ICC/IHC, western blotting, and ELISAs. Check out these recent blog posts, showcasing the latest antibody data for selected nucleic acid and protein research topics:
- Translate to Invitrogen antibodies for your ribosomal protein research!
- Drivers of the chromosomal passenger complex
- Pairing Invitrogen ChIP-validated* antibodies with your DNA repair protein research
Find these posts and more in the Behind the Bench Blog
*The use or any variation of the word “validation” refers only to research use antibodies that were subject to functional testing to confirm that the antibody can be used with the research techniques indicated. It does not ensure that the product(s) was validated for clinical or diagnostic uses.
Introducing BlotBuilder: A new western blot product selection tool
Imagine if you could obtain a personalized western blot solution designed specifically for your experimental needs—in just 2 minutes. Now you can with our new Invitrogen BlotBuilder Western Blot Product Selection Tool, which can help you quickly and easily find the optimal set of western blot products for detection of your protein. Simply answer 5 questions and get a customized western blot protocol and product list based on your needs.
Customize your blot experience today with our BlotBuilder tool
Best practices: 5 steps to intracellular flow cytometry
The ability to detect intracellular proteins with flow cytometry opens the door to a more complete characterization of cell populations and distinct subsets within those populations. To help you save time and effort and increase your efficiency when designing flow cytometry experiments, we have organized our resources, tools, and products for intracellular flow cytometry into 5 simple steps:
- Step 1: Target determination, with a link to our antibody search tool
- Step 2: Buffer selection, with fixation and permeabilization buffers for cytoplasmic and nuclear staining
- Step 3: Panel design, with online tools to create a customized panel and search published multicolor flow cytometry panels
- Step 4: Biological controls, with a product selection guide that contains fluorescent cell stains for removing dead cells and unwanted artifacts from your analysis
- Step 5: Experimental controls, with products to facilitate antibody compensation controls
Access these resources at "5 steps to intracellular flow cytometry"
For Research Use Only. Not for use in diagnostic procedures.