Our Newest Cellular Analysis Products and Technologies
- ABfinity™ Recombinant Monoclonal Antibodies
- ABfinity™ Anti-GFP Antibody For Multiple Applications
- New Packaging For Selected Molecular Probes® Products
- NovaBright™ SEAP Enzyme Reporter Gene Assay
- BrdU Mouse Monoclonal Antibody, Clone MoBU-1
- Autophagy Imaging Tools
- New Recombinant Frizzled And VEGF Proteins
- Learn more about ABfinity™ Antibodies
Lot-to-lot consistency of western blotting results with ABfinity™ antibody..
- Learn More about our Anti-GFP Antibodies
Anti-GFP ABfinity™ Recombinant Rabbit Monoclonal Antibody used for immunocytochemistry. (Left) U2OS cells expressing Organelle Lights™ ER-GFP were analyzed using the anti-GFP ABfinity™ Recombinant Rabbit Monoclonal Antibody. (Middle) Cells were formaldehyde-fixed, permeabilized, and blocked in 1% BSA, then incubated with primary antibody at 1 μg/mL, followed by Alexa Fluor® 647 goat anti–rabbit IgG conjugate. Nuclei were stained with Hoechst 33342. (Right) The merged yellow signal indicates colocalization of GFP fluorescence and the detection antibody.
When you purchase selected Molecular Probes® products, you’ll notice less packaging but the same uncompromised performance. We are committed to offering you a simple way to contribute to environmental sustainability through the responsible use and disposal of packaging in your everyday research. Selected kits and assays are packaged in lightweight pouches instead of jars. This change has resulted in a reduction of material weight by 73%, a reduction of storage and shipping space by up to 68%, and elimination of nonrecyclable foam inserts. As a leading life science research company, we will continue our efforts to reduce our environmental impact. Life Technologies is a member of the FTSE4Good Index Series and the Dow Jones Sustainability Index, where it was Biotechnology Sector Leader in 2008.
Detection of secreted alkaline phosphatase (SEAP) reporter gene activity is easier than ever with the NovaBright™ SEAP 2.0 reporter gene assay. By utilizing our high-performance alkaline phosphatase substrate, CSPD®, and our next-generation Emerald™ enhancer, better assay performance is obtained over 5 orders of magnitude of enzyme concentration. The assay has been simplified down to two ready-to-use assay reagents, eliminating reagent preparation and sample dilution steps. Our proprietary buffer formulation ensures low background from endogenous phosphatases, so NovaBright™ SEAP 2.0 enables the most sensitive detection by providing a higher signal-to-noise ratio of SEAP reporter gene activity than other assays of its kind.
- Learn more about NovaBright™ SEAP Enzyme Reporter Gene Assay
Cell proliferation can be measured by following the incorporation of the thymidine analog BrdU into newly synthesized DNA, and subsequent detection with an anti-BrdU antibody. The MoBU-1 clone has high specificity for BrdU while displaying no cross-reactivity with EdU, thus making it valuable for use in dual pulse applications. The MoBU-1 clone is available as a direct conjugate for flow cytometry for use with the violet, blue, and red lasers. Unconjugated, biotinylated, and direct conjugates with photostable Alexa Fluor® dyes have also been validated for use in immunofluorescence microscopy and high-content applications.
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Labeling of cell proliferation using BrdU and BrdU Mouse Monoclonal Antibody conjugates. Jurkat T cell leukemia cells were treated with 10 μM BrdU for 1.5 hr. Cells were then fixed in ethanol and stored overnight at ≤–20°C. An acid denaturation method was used before labeling 1 x 106 cells with 5 μL of the following BrdU Mouse Monoclonal Antibody (Clone MoBU-1) conjugates: (A) Pacific Blue™ conjugate, (B) Alexa Fluor® 488 conjugate, and (C) Alexa Fluor® 647 conjugate. Proliferating cells are clearly distinguished from nonproliferating cells.
- Learn more about Autophagy Imaging Tools
The Frizzled family of transmembrane proteins serves as receptors for the Wnt signaling pathway. Wnt signaling is involved in various developmental processes, including cell polarity, cell fate determination, and the control of cell proliferation. The Frizzled proteins have been implicated in both the canonical Wnt/β-catenin pathway and the Wnt/Ca2+ flux pathway. We now offer bioactive recombinant Frizzled 1, Frizzed 4, and Frizzled 7. Recombinant human vascular endothelial growth factor (VEGF) is a bioactive protein intended for use in cell culture applications. VEGF exerts angiogenic, mitogenic, and vascular permeability–enhancing activities specific for endothelial cells.