ABfinity™ Recombinant Monoclonal Antibodies

ABfinity™ antibodies, produced using proprietary Invitrogen™ technology, are highly specific, high-quality monoclonal antibodies that are unmatched in producing consistent results. ABfinity™ antibodies are recombinant rabbit monoclonal antibodies developed by immunizing animals, screening for functionality, and cloning the immunogen-specific antibody genes into high-level expression vectors. The antibodies are produced on a large scale by expressing them in mammalian cells and purifying them with protein A. This highly reproducible process results in unparalleled lot-to-lot consistency.


Lot-to-lot consistency of western blotting results with ABfinity™ antibody.  

Lot-to-lot consistency of western blotting results with ABfinity™ antibody.


ABfinity™ Anti-GFP Antibody For Multiple Applications

The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is a versatile marker for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression. But why use different anti-GFP antibodies for different applications? Invitrogen’s new anti-GFP ABfinity™ Recombinant Rabbit Monoclonal Antibody detects native GFP, GFP variants, and most GFP fusion proteins in multiple applications, including western blots, immunoprecipitation, ELISA, flow cytometry, immunohistochemistry, and immunocytochemistry. It’s a simple choice for all your GFP detection needs.


Anti-GFP ABfinity™ Recombinant Rabbit Monoclonal Antibody used for immunocytochemistry.

Anti-GFP ABfinity™ Recombinant Rabbit Monoclonal Antibody used for immunocytochemistry.
(Left) U2OS cells expressing Organelle Lights™ ER-GFP were analyzed using the anti-GFP ABfinity™ Recombinant Rabbit Monoclonal Antibody. (Middle) Cells were formaldehyde-fixed, permeabilized, and blocked in 1% BSA, then incubated with primary antibody at 1 μg/mL, followed by Alexa Fluor® 647 goat anti–rabbit IgG conjugate. Nuclei were stained with Hoechst 33342. (Right) The merged yellow signal indicates colocalization of GFP fluorescence and the detection antibody.

New Packaging For Selected Molecular Probes® Products

When you purchase selected Molecular Probes® products, you’ll notice less packaging but the same uncompromised performance. We are committed to offering you a simple way to contribute to environmental sustainability through the responsible use and disposal of packaging in your everyday research. Selected kits and assays are packaged in lightweight pouches instead of jars. This change has resulted in a reduction of material weight by 73%, a reduction of storage and shipping space by up to 68%, and elimination of nonrecyclable foam inserts. As a leading life science research company, we will continue our efforts to reduce our environmental impact. Life Technologies is a member of the FTSE4Good Index Series and the Dow Jones Sustainability Index, where it was Biotechnology Sector Leader in 2008.

NovaBright™ SEAP Enzyme Reporter Gene Assay

Detection of secreted alkaline phosphatase (SEAP) reporter gene activity is easier than ever with the NovaBright™ SEAP 2.0 reporter gene assay. By utilizing our high-performance alkaline phosphatase substrate, CSPD®, and our next-generation Emerald™ enhancer, better assay performance is obtained over 5 orders of magnitude of enzyme concentration. The assay has been simplified down to two ready-to-use assay reagents, eliminating reagent preparation and sample dilution steps. Our proprietary buffer formulation ensures low background from endogenous phosphatases, so NovaBright™ SEAP 2.0 enables the most sensitive detection by providing a higher signal-to-noise ratio of SEAP reporter gene activity than other assays of its kind.


NovaBright™ SEAP Enzyme Reporter Gene Assays
   NovaBright™ SEAP 2.0 assay kinetics compared to other SEAP reporter gene kits. pCMV-transfected NIH/3T3 cells were assayed with NovaBright™ SEAP 2.0 (blue), NovaBright™ SEAP (red), supplier A (green), or supplier B (orange) assay kits. NovaBright™ SEAP and SEAP 2.0 clearly enable detection of secreted placental alkaline phosphatase with lower limits of detection and higher signal to noise than the other commercially available kits.

BrdU Mouse Monoclonal Antibody, Clone MoBU-1

Cell proliferation can be measured by following the incorporation of the thymidine analog BrdU into newly synthesized DNA, and subsequent detection with an anti-BrdU antibody. The MoBU-1 clone has high specificity for BrdU while displaying no cross-reactivity with EdU, thus making it valuable for use in dual pulse applications. The MoBU-1 clone is available as a direct conjugate for flow cytometry for use with the violet, blue, and red lasers. Unconjugated, biotinylated, and direct conjugates with photostable Alexa Fluor® dyes have also been validated for use in immunofluorescence microscopy and high-content applications.


Anti-GFP ABfinity™ Recombinant Rabbit Monoclonal Antibody used for immunocytochemistry.
(click to enlarge)

Labeling of cell proliferation using BrdU and BrdU Mouse Monoclonal Antibody conjugates. Jurkat T cell leukemia cells were treated with 10 μM BrdU for 1.5 hr. Cells were then fixed in ethanol and stored overnight at ≤–20°C. An acid denaturation method was used before labeling 1 x 106 cells with 5 μL of the following BrdU Mouse Monoclonal Antibody (Clone MoBU-1) conjugates: (A) Pacific Blue™ conjugate, (B) Alexa Fluor® 488 conjugate, and (C) Alexa Fluor® 647 conjugate. Proliferating cells are clearly distinguished from nonproliferating cells.

Autophagy Imaging Tools

The Premo™ Autophagy Sensor combines the selectivity of an LC3B–fluorescent protein (LC3B-FP) chimera with the transduction efficiency of BacMam technology, enabling unambiguous visualization of this protein. Unlike expression vectors, BacMam reagents enable titratable and reproducible expression and offer high cotransduction efficiency. Recent improvements to the BacMam system enable efficient transduction in a wider variety of cells, including neurons and neural stem cells, with an easy one-step protocol. Each Premo™ Autophagy Sensor kit includes a BacMam LC3B-FP, a control BacMam LC3B (G120A)-FP, and chloroquine diphosphate to artificially induce autophagosome accumulation. The LC3B Antibody Kit for Autophagy includes a rabbit polyclonal antibody against LC3B that has been validated for use in fluorescence microscopy and high-content imaging and analysis. Chloroquine diphosphate is also included; following treatment with this compound, normal autophagic flux is disrupted and autophagosomes accumulate as a result of increased lysosomal pH.

New Recombinant Frizzled And VEGF Proteins

The Frizzled family of transmembrane proteins serves as receptors for the Wnt signaling pathway. Wnt signaling is involved in various developmental processes, including cell polarity, cell fate determination, and the control of cell proliferation. The Frizzled proteins have been implicated in both the canonical Wnt/β-catenin pathway and the Wnt/Ca2+ flux pathway. We now offer bioactive recombinant Frizzled 1, Frizzed 4, and Frizzled 7. Recombinant human vascular endothelial growth factor (VEGF) is a bioactive protein intended for use in cell culture applications. VEGF exerts angiogenic, mitogenic, and vascular permeability–enhancing activities specific for endothelial cells.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.