Our newest cell and protein biology products and technologies

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Elevate your images with Alexa Fluor Plus secondary antibodies

Visualize your low-abundance targets, spend less time optimizing signals, and make every one of your samples count with Invitrogen Alexa Fluor Plus secondary antibodies. These fluorescent antibody conjugates provide up to 5.8 times higher signal-to-noise ratios than traditional Alexa Fluor secondary antibodies, due to both the proprietary formulation of the Alexa Fluor Plus dyes and preadsorption of the antibodies to minimize cross-reactivity. With the set of 36 Alexa Fluor Plus secondary antibodies, you can now perform enhanced multiplex experiments, mixing and matching the six species and target combinations or combining them with traditional Alexa Fluor antibody conjugates for full spectral coverage. In addition, we have recently improved the original Alexa Fluor 594 dye and conjugated it to six of our best secondary antibodies, enabling more sensitive red-fluorescent detection in immunofluorescence (IF) applications. The Alexa Fluor Plus secondary antibodies are recommended for imaging and western blotting.

Learn more about Alexa Fluor Plus secondary antibodies

Tubulin detection with either Alexa Fluor 594 or Alexa Fluor Plus 594 conjugates

Tubulin detection with either Alexa Fluor 594 or Alexa Fluor Plus 594 conjugates. An Alexa Fluor 594 secondary antibody from another supplier (left) or Invitrogen Alexa Fluor Plus 594 donkey anti–mouse IgG secondary antibody (right) was used for immunofluorescent detection of tubulin (red) in U2OS cells. Nuclei were counterstained with Invitrogen NucBlue Live ReadyProbes Reagent (blue), and images were obtained using the Invitrogen EVOS FL Auto 2 Imaging System with a 20x objective.

Secondary antibody Alexa Fluor Plus 488 Alexa Fluor Plus 555 Alexa Fluor Plus 594 Alexa Fluor Plus 647 Alexa Fluor Plus 680 Alexa Fluor Plus 800
Goat anti–rabbit IgG A32731 › A32732 › A32740 › A32733 › A32734 › A32735 ›
Goat anti–mouse IgG A32723 › A32727 › A32742 › A32728 › A32729 › A32730 ›
Goat anti–chicken IgY A32931 › A32932 › A32759 › A32933 › A32934 › A32935 ›
Donkey anti–goat IgG A32814 › A32816 › A32758 › A32849 › A32860 › A32930 ›
Donkey anti–rabbit IgG A32790 › A32794 › A32754 › A32795 › A32802 › A32808 ›
Donkey anti–mouse IgG A32766 › A32773 › A32744 › A32787 › A32788 › A32789 ›

The future is now with ProQuantum high-sensitivity immunoassays

The Invitrogen ProQuantum high-sensitivity immunoassay platform is an affordable, next-generation, no-wash method for cytokine detection and quantitation that requires only very small sample volumes. Collecting samples from mouse models can be particularly challenging. While conventional ELISAs typically require 50 μL of serum per well, the ProQuantum Immunoassay Kits only use 2 μL of serum to obtain results in duplicate or even triplicate. The small sample requirements combined with the high sensitivity and large dynamic range inherent in real-time PCR amplification make ProQuantum immunoassays the go-to solution when you need to find that needle-in-a-haystack analyte in your mouse serum samples.

Learn more about ProQuantum Immunoassay Kits

Standard curve for the IFN gamma Mouse ProQuantum Immunoassay Kit

Standard curve for the IFN gamma Mouse ProQuantum Immunoassay Kit. This standard curve shows an assay range of 0.064–5,000 pg/mL.


Flow cytometry antibodies: More options to fit your research

At Thermo Fisher Scientific, we understand the need for choice when selecting antibodies for multicolor flow cytometry panels, and we continue to release new conjugates every month. For example, we have recently expanded our macrophage marker portfolio. Highlights include antibodies that recognize arginase-1, RELM alpha, CD163, MARCO, AXL, and CXCL13, several of which are discussed in Invitrogen Behind the Bench blog posts. In addition, the growing portfolio of Super Bright antibodies now includes nearly 700 antibody conjugates labeled with one of the five Super Bright dyes— Super Bright 436, Super Bright 600, Super Bright 645, Super Bright 702, and Super Bright 780—all of which are compatible with excitation from the violet laser.

Choose from our wide selection oft flow cytometry antibodies

Immunophenotyping with anti–mouse RELM alpha antibody

Immunophenotyping with anti–mouse RELM alpha antibody. C57BL/6 mouse resident peritoneal exudate cells were surface-stained with Invitrogen F4/80 monoclonal antibody (clone BM8), eFluor 450, fixed, permeabilized, and then intracellularly stained with either Invitrogen Rat IgG1 kappa Isotype Control (clone eBRG1), APC (left); or Invitrogen RELM alpha Monoclonal Antibody (clone DS8RELM), APC (right). All peritoneal cells were used for analysis; the majority of double-negative cells are B cells.


ProcartaPlex multiplex immunoassays for cardiovascular research

Cardiovascular diseases (CVDs), which are disorders of the heart and blood vessels, are considered to be the major cause of death globally. Cardiovascular research is mainly focused on understanding the complex molecular interactions and regulatory mechanisms involved in normal and pathological cardiovascular states. Newly released Invitrogen ProcartaPlex Panels for the Luminex® xMAP® instrument platform allow researchers to simultaneously study multiple protein biomarkers relevant to cardiac disease, cardiac remodeling, arteriosclerosis, atherosclerosis, and angiogenesis in human serum, plasma, and cell culture supernatant samples.

Learn more about ProcartaPlex Panels

Detection of troponin I using the ProcartaPlex immunoassay

Detection of troponin I using the ProcartaPlex immunoassay. Troponin I levels in human plasma from patients with different cardiovascular diseases were measured using the Invitrogen Cardiac Disease 9-Plex Human ProcartaPlex Panel.


More sensitive cell viability assays: Introducing alamarBlue HS and PrestoBlue HS reagents

All resazurin-based cell viability reagents contain a detectable amount of intensely fluorescent resorufin (the oxidized product of resazurin), which contaminates the assay and leads to increased background signal and reduced sensitivity. Using an innovative process to purify resazurin and remove contaminants, we have developed the Invitrogen alamarBlue HS and PrestoBlue HS Cell Viability Reagents. Formulated with highly purified resazurin, these high-sensitivity (HS) reagents enable:

  • >50% reduction in background, due to contaminant removal
  • >100% increase in signal-to-background ratios, producing a linear response and a large assay window
  • Detection of as few as 20 cells per well
  • Convenient add-and-read format, with no mixing, washing, or cell lysis required
  • Analysis on either fluorescence- or absorption-based instrumentation

Both alamarBlue HS and PrestoBlue HS Cell Viability Reagents are nontoxic, aseptic reagents that do not require cell lysis. Therefore, once the assay is completed, the diluted alamarBlue HS and PrestoBlue HS reagents can be removed and replaced with complete growth medium to allow cells to be further cultured. PrestoBlue HS reagent is recommended for quick viability determination, whereas alamarBlue HS reagent is recommended in cases of extended viability studies or when using a high cell density.

Learn more about microplate assays for cell viability

Sensitivity comparison between conventional cell viability reagents and the new alamarBlue HS and PrestoBlue HS reagents. A549 cells were assayed with the indicated cell viability reagents according to the manufacturers’ protocols. Resorufin fluorescence was measured using the Thermo Scientific Varioskan LUX Multimode Microplate Reader.


Super new look. Same SuperSignal product.

Thermo Scientific SuperSignal chemiluminescent horseradish peroxidase (HRP) substrates are getting a new look, but the products inside are still the same high-quality substrates, developed and manufactured by the same dedicated experts you have trusted for years. The new flip-top box design allows reagent bottles to stand upright. Additionally, the clear bottle has a transparent label so you can easily see the volume remaining.

Learn more about chemiluminscent western blot detection

SuperSignal West Pico PLUS Chemiluminescent Substrate

SuperSignal West Pico PLUS Chemiluminescent Substrate.

 


The powerful, new EVOS M7000 Imaging System

The Invitrogen EVOS M7000 Imaging System is a hardware refresh of the popular and fully automated Invitrogen EVOS FL Auto 2 Imaging System, with upgraded cameras that yield higher sensitivity and resolution and a high-performance PC for faster image acquisition and data processing. It was developed to meet today’s expectations for image quality, user–interface interactions, speed of data generation, and flexibility. The automated image acquisition tools with enhanced autofocus algorithms are designed for demanding applications, including 3D imaging, time-lapse experiments, and large tile-stitch samples. With the optional Invitrogen Celleste 5.0 Image Analysis Software, the EVOS M7000 system becomes an exceptionally powerful tool for image acquisition, analysis, and rendering.

Learn more about the EVOS M7000 Imaging System

EVOS M7000 Imaging System

EVOS M7000 Imaging System.


The EVOS M5000 Imaging System: A fluorescence imaging workhorse

Released in late 2018, the Invitrogen EVOS M5000 Imaging System has already generated tremendous interest among researchers who need a robust, flexible, and easy-to-use microscope. It is a fully integrated digital inverted benchtop microscope that affords 4-color fluorescence, transmitted-light, and color imaging. With interchangeable optics and autofocus, single-click multichannel image acquisition, and an interface designed for usability, the EVOS M5000 system allows you to acquire publication-quality images in minutes. In addition, post-capture tools—from brightness/contrast to annotations and automated cell counting and confluence measurements—make it an exceptionally versatile microscope that is ideal for a broad range of imaging applications.

Learn more about the EVOS M5000 Imaging System

EVOS M5000 Imaging System

EVOS M5000 Imaging System.


EVOS long working distance objectives

Together with the Invitrogen EVOS M7000 system, we introduced a line of long working distance (LWD) objectives designed for 1.0 mm–thick sample vessels. These objectives are available in achromat and fluorite glass quality, with phase options for both. They are ideal for imaging cells on most microscope slides and some plasticware. As with all EVOS objectives, they offer outstanding optical performance with light from visible to near-infrared. Together with the existing EVOS LWD objectives designed for 1.2 mm–thick sample vessels, these objectives offer more choices to provide the best image quality, particularly at higher magnifications of 40x and 60x.

Learn more about EVOS long working distance objectives

EVOS long working distance (LWD) objectives

EVOS long working distance (LWD) objectives.