SlowFade Glass Antifade Mountants: Unparalleled focal depth

Obtain the sharpest deep-tissue images with Invitrogen SlowFade Glass Soft-Set Antifade Mountant, which features a refractive index of 1.52 (similar to glass coverslips) and provides superior photobleaching protection. This noncuring mountant is compatible with oil-immersion microscope optics and enables a refraction-free light path and minimized spherical aberration, which improves axial resolution threefold (at 150 μm focal depth) as measured by point spread function. When 1 mm–thick tissue sections are mounted in SlowFade Glass Soft-Set Antifade Mountant, there is visible optical tissue clearing, enabling deep-tissue 3D imaging.

Learn more about our growing portfolio of noncuring and hard-set antifade mountants

immunofluorescent brain tissue sections mounted in SlowFade Glass showing improved focal depth (axial resolution)

Unparalleled focal depth in 100 μm–thick brain tissue sections with SlowFade Glass Soft-Set Antifade Mountant. Cryopreserved 100 μm–thick rat brain sections were stained for GFAP (red) with Invitrogen GFAP rabbit primary antibody and Invitrogen Alexa Fluor Plus 594 goat anti–rabbit IgG secondary antibody overnight. Nuclei (cyan) were stained with Invitrogen DAPI nuclear stain. Stained tissue sections were mounted with Invitrogen SlowFade Glass Soft-Set, Invitrogen SlowFade Diamond, or Invitrogen SlowFade Gold Antifade Mountant and imaged on a Zeiss LSM 710 confocal microscope.

Alexa Fluor Plus Phalloidins for superior actin cytoskeleton imaging

Invitrogen Alexa Fluor Plus 555 and Alexa Fluor Plus 647 Phalloidins are high-affinity F-actin probes conjugated to our bright and photostable Alexa Fluor Plus dyes. These phalloidin conjugates are specially designed to produce the maximum density of both phalloidin and Alexa Fluor Plus dye molecules on an F-actin filament, resulting in 3 to 5 times more fluorescent signal for superior actin cytoskeleton imaging.

The probes are designed to produce excellent sensitivity and specificity when visualizing and quantifying F-actin in cells, tissue sections, or cell-free preparations. Their extra brightness is especially useful when conducting challenging F-actin imaging, such as structured illumination microscopy (SIM) or stochastic optical reconstruction microscopy (STORM), or for reliable staining of stress fibers. Phalloidin-based actin staining is fully compatible with other fluorescent stains used in cell analyses.

Learn more about our phalloidin conjugates for actin staining

immunofluorescent HeLa cell stained with Alexa Fluor Plus 555 phalloidin and DAPI

CountBright Plus beads: Absolute cell counting with greater accuracy and ease

Absolute cell counting beads are widely used in flow cytometry experiments to quantify cell populations and study disease progression. They can also be used to validate flow cytometric absolute cell counting results. Compared with the original Invitrogen CountBright Absolute Counting Beads, the new Invitrogen CountBright Plus beads offer: (1) a greater percentage of singlets for improved accuracy and consistency, and (2) excitation by a wider range of wavelengths, from ultraviolet (350 nm) to infrared (808 nm), with emissions between 385 and 860 nm. The CountBright Plus beads also have a 4 μm diameter, which is similar to that of blood cells and enables cell counting beads to be on scale for FSC/SSC graphs.

Find out more about our selection of flow cytometry cell counting beads

flow cytometry plot of count versus fluorescence showing simultaneous detection of CountBright Plus beads and CD19+ cells

Simultaneous detection of CountBright Plus Absolute Counting Beads and near-IR–emitting fluorophores. Invitrogen CountBright Plus Absolute Counting Beads are detected simultaneously with Invitrogen APC–eFluor 780 CD19 antibody–stained cells in lysed whole blood excited with an infrared laser.

TMTpro 16plex Isobaric Label Reagents: Next-generation tandem mass tags

Thermo Scientific TMTpro 16plex Isobaric Label Reagents represent the next generation of tandem mass tags for mass spectrometry. They are designed to increase the level of sample multiplexing without compromising either protein identification or quantitation. These new TMTpro label reagents have the same labeling efficiency and peptide/protein ID rates and are similar in design to the original Thermo Scientific TMT label reagents (i.e., they are both isobaric and amine-reactive); however, they differ in structure, with a longer spacer region and an isobutyl proline mass reporter region.

The higher multiplex capability provided by the TMTpro label reagents results in fewer missing quantitative values among samples and within replicates. These new tandem mass tags are designed to produce superior quantitative accuracy and precision.

Find out more about our selection of tandem mass tag (TMT) systems

TMTpro structure noting mass reporter, cleavable linker, mass normalizer, and amine-reactive group

The general chemical structure of the TMTpro isobaric label reagents.

ProcartaPlex multiplex immunoassay panels for neuroscience research

Each step of the protein expression and purification workflow can be time-consuming, taking days to weeks to complete. Moreover, traditional methods used to confirm protein expression require significant hands-on time. Thermo Scientific Pro-Detect Rapid Assay Kits enable quick and efficient detection of tagged recombinant proteins without the need to perform complex assays such as ELISA or western blotting. Pro-Detect Rapid Assay Kits are single-use, dipstick-style lateral flow strips that provide qualitative confirmation of expressed fusion-tag proteins in 10–15 minutes. These rapid test kits can detect a specific protein tag of interest—e.g., His, GST, Myc, and Fc—directly from cell culture media, cell lysates, or purified protein solutions, and display the results visually without the need for specialized equipment.

Learn more about Pro-Detect Rapid Assay Kits

Elements of the central nervous system. The Invitrogen Neuroscience 18-plex Human ProcartaPlex Panel can be used to simultaneously measure 18 different markers in biofluids such as cerebrospinal fluid or blood that play a role in neuroinflammation, neurodegeneration, and traumatic brain injury.

ProQuantum high-sensitivity immunoassays: Now over 60 available targets

The Invitrogen ProQuantum high-sensitivity immunoassay kits offer affordable, ready-to-use, qPCR-based protein quantitation that consume as little as 2 μL of sample for triplicate data (a typical ELISA requires 150 μL). The assay protocol is streamlined with no wash steps, a single 1-hour incubation, and results in only 2 hours. With qPCR amplification, this immunoassay provides a very broad dynamic range (≥5 orders of magnitude) and high sensitivity, giving you the best chances of detecting the protein of interest.

Learn more about ProQuantum high-sensitivity immunoassays

line graph of Ct (cycle threshold)  versus IL-6 concentration using ProQuantum kit shows assay range >5 orders of magnitude

Standard curve for the ProQuantum Mouse IL-6 Immunoassay Kit. This standard curve, obtained using the Invitrogen ProQuantum Mouse IL-6 Immunoassay Kit, shows an assay range of 0.32–25,000 pg/mL.

Innovative chemistry in the new Click-iT EdU Proliferation Assay for Microplates

Measuring mammalian cell proliferation is a fundamental method for assessing cell health, determining genotoxicity, and evaluating anti-cancer drugs. The new-and-improved Invitrogen Click-iT EdU Proliferation Assay for Microplates directly detects and quantifies DNA synthesis using a simplified and robust method that relies on click chemistry.

After EdU (a modified thymidine analog) is incorporated into newly synthesized DNA, a horseradish peroxidase (HRP) azide derivative is covalently attached to the incorporated EdU via a highly specific click reaction. The Invitrogen Amplex UltraRed Reagent, an HRP substrate included in this kit, is then added and the resulting fluorescence signal detected. Because of the highly specific attachment of HRP to EdU, the assay provides a highly sensitive and reliable measurement of mammalian cell proliferation in a microplate format. Furthermore, compared with the previous version of this assay, the new Click-iT EdU Proliferation Assay for Microplates shows very significant increases in sensitivity and assay dynamic range.

Learn more about our full range of Click-iT Assay Kits

line graphs of cell fluorescence versus nocodazole concentration showing better sensitivity with new Click-iT EdU Proliferation Assay

Significant performance improvement demonstrated using the new Click-iT EdU Proliferation Assay for Microplates. HeLa cells were seeded into 96-well plates, incubated overnight to allow for attachment, and then treated with various concentrations of nocodazole, which interferes with microtubule polymerization leading to an arrest of mitosis. After drug treatment, EdU was added, the cells were incubated for 2 hr, and cell proliferation was detected using the previous version (Cat. No. C10214, now discontinued) and the new version of the Invitrogen Click-iT EdU Proliferation Assay for Microplates.

SiteClick Antibody Labeling Kits: Now available in large pack sizes

The Invitrogen SiteClick Antibody Labeling Kits, which produce labeled primary antibodies using highly efficient, site-specific, click labeling chemistry, are now available in large pack sizes for labeling up to 5 mg of antibody (in addition to the current pack size for 100 μg antibody). The SiteClick system allows simple and gentle site-selective attachment of detection molecules to heavy chain N-linked glycans (far from the antigen-binding domain) of primary antibodies, providing excellent reproducibility from labeling to labeling and antibody to antibody. Once the antibody’s glycans are azido modified, a variety of detection molecules (Invitrogen SiteClick sDIBO alkynes)—including phycobiliproteins (e.g., R-PE), fluorescent dyes, Invitrogen Qdot probes, metal-chelating compounds, and other small molecules like biotin—are available for attachment to the antibody via click chemistry, allowing multiplex analysis with antibodies from the same species.

Learn more about our wide selection of antibody labeling kits

line graph showing more signal with SiteClick label versus amine-reactive label and histogram showing labeling consistency

SiteClick antibody labeling. (A) Invitrogen SiteClick antibody labeling vs. amine-reactive labeling in a bead-based sandwich assay. (B) Demonstration of antibody-to-antibody consistency with SiteClick antibody labeling.

Invitrogen H3K4me3 antibody with demonstrated specificity in ChIP

Histone modifications serve as epigenetic signatures for gene expression. In partnership with EpiCypher Inc., we are using SNAP-ChIP (Sample Normalization and Antibody Profiling for Chromatin Immunoprecipitation) reagents to validate histone antibodies for specificity in ChIP applications. SNAP-ChIP Spike-In Controls are a panel of recombinant barcoded nucleosomes that are spiked into a ChIP workflow. qPCR amplification of the barcodes allows determination of the pull-down efficiency of the antibody and whether it recognizes any off-target modifications (demonstrated here with the Invitrogen H3K4me3 antibody).

Learn more about our advanced verification methods and search our complete antibody database

histogram showing SNAP-ChIP assay for H3K4me3 antibody to verify histone modification specificity

Histone modification specificity analysis. The EpiCypher SNAP-ChIP K-MetStat Panel was used to analyze the performance of Invitrogen H3K4me3 recombinant polyclonal antibody in ChIP. Specificity (left y-axis) was determined by qPCR for each modified nucleosome in the SNAP-ChIP panel (x-axis). Green bar represents antibody efficiency and indicates percentage of the barcoded nucleosome target immunoprecipitated relative to input. All bars represent mean ± SEM.

New Hepatic Spheroid Kit with 3D-qualified hepatocytes

3D cell models have become important experimental tools for cancer biology and immunology, as well as for drug screening, discovery, and development. These models—including spheroids, tumor spheroids or tumoroids, and organoids—are relevant for many application areas because their microenvironment can mimic that of in vivo systems better than traditional 2D models. We now offer Gibco human spheroid-qualified hepatocytes to facilitate the production of 3D human liver models. When compared with traditional 2D hepatic cell cultures, hepatic spheroids generated using the Gibco Hepatic Spheroid Kit demonstrate:

  • Increased cell-to-cell interactions
  • Superior longevity (>21 days, 3 times longer than most 2D hepatic cultures)
  • Improved hepatocyte-specific function (e.g., higher levels of albumin, CYP, and MDR1 expression)

The Hepatic Spheroid Kit provides human spheroid-qualified hepatocytes, as well as media, supplements, and cultureware for 3D culture.

Review our application data for the Gibco human spheroid-qualified hepatocytes

New Pierce Dye and Biotin Removal Spin Columns and Filter Plates

Removing free dye from a protein sample after a labeling reaction is often difficult and time-consuming, but it is essential for accurate determination of dye-to-protein ratios. Thermo Scientific Pierce Dye and Biotin Removal Spin Columns and Filter Plates are highly specialized to produce exceptional protein recovery (for proteins >7 kDa), while quickly and effectively removing nonconjugated fluorescent dyes, biotinylation reagents, reducing agents, and crosslinkers. Product highlights include:

  • High recovery—low-binding resin maximizes protein recovery
  • Ease-of-use—no cumbersome column preparation or equilibration
  • Fast—dye removal and protein recovery in less than 15 minutes

Learn more about protein sample cleanup