How Luminex® Technology Works

The Luminex® xMAP® technology is based on polystyrene or paramagnetic microspheres, or beads, that are internally dyed with red and infrared fluorophores of differing intensities. Each dyed bead is given a unique number, or bead region, allowing the differentiation of one bead from another. For Novex® multiplex immunoassay kits, individual bead sets are then coated with a capture antibody qualified for one specific analyte (Figure 4). Multiple analyte-specific beads can then be combined in a single well of a 96-well microplate-format assay to detect and quantify multiple targets simultaneously, using one of the Luminex® instruments for analysis. We offer multiplex assays using either polystyrene or paramagnetic beads.

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Protocol used with bead-based assays (Luminex® Corporation).