Factors Influencing Protein Quantitation
Novex® singleplex and multiplex assays, based on Luminex® xMAP® technology, provide a versatile platform that gives users more flexibility and a greater array of options for measuring single or multiple analytes. The new line of paramagnetic bead assays was carefully designed and tested to help ensure that sensitivity, range, and correlation to other assay systems are maximized, using some of the same components as our polystyrene bead assays. This means that the paramagnetic and polystyrene bead–based assays perform with comparable quality and consistency (Figure 1).
Advantages of Novex® paramagnetic bead assay kits include:
- Compatibility with manual or automated handling
- Easy wash steps with the handheld 96-well magnetic separator (Figure 1)
- Automation-enabled wash steps that eliminate vacuum manifold washes, clogging of filter plates, and bead loss
Luminex® systems are built on proven technology—flow cytometry, microspheres, lasers, digital signal
processing, and traditional chemistry—combined in a unique way. Featuring a flexible open-architecture
design, Luminex® xMAP® technology can perform a wide variety of bioassays quickly, cost-effectively, and
accurately. In Table 3, we present an overview of the 3 different Luminex® instruments available for multiplex
detection and analysis.
Table 1. Overview of the Luminex® instrument systems.
||Luminex® 100/200™ systemFLEXMAP 3D® system
||FLEXMAP 3D® system
||Affordable, efficient, compact size||Versatile and efficient; the standard in multiplexing||High-throughput, high-plex,
||Fluorescence imager||Flow cytometry–based||Flow cytometry–based|
||Magnetic beads only||Polystyrene and magnetic beads||Polystyrene and magnetic beads|
|Read time for 96-well plate
||~60 min||~40 min||~20 min|
||3.5 logs||3.5 logs||4.5 logs|
||96-well||96-well||96-well and 384-well|
The MAGPIX® system analyzes magnetic beads immobilized with a magnet, excites the beads using lightemitting
diodes (LEDs), and then detects and analyzes the beads using a CCD camera (Figure 3). The MAGPIX® system is thus a more compact, robust, and cost-effective multiplexing tool. In addition, streamlined startup and shutdown protocols, and minimal maintenance requirements make the system easy to use—ideal for both new and experienced users. The MAGPIX® system is a compact benchtop instrument with a multiplex capability of up to 50 analytes. It features simple out-of-the-box setup and interactive software. For more details, please contact your Life Technologies sales representative or visit lifetechnologies.com/magpix.
The Luminex® 100/200™ system is a compact analyzer that performs up to 100 assays simultaneously in a single well of a microtiter plate. This flexible system is based on the principles of flow cytometry, integrating key xMAP® detection components to meet the multiplex testing needs of research laboratory professionals. For more details, please contact your Life Technologies sales representative or visit our Luminex® Instruments page for additional information.
The FLEXMAP 3D® system combines differentially dyed fluorescent microsphere sets with an innovative instrument design to take bead-based multiplexing technology to the next dimension, and is capable of multiplexing up to 500 analytes simultaneously. For more details, please contact your Life Technologies sales representative or visit lifetechnologies.com/luminexinstrument. The Luminex® 100/200™ and FLEXMAP 3D® systems have gained wide acceptance for use in applications such as protein expression profiling, focused gene expression profiling, and disease testing. These instruments perform sample analysis using flow cytometry, exciting samples with two lasers, and evaluating the results using real-time digital signal processing and xPONENT® software to distinguish bead color (analyte) and assay signal-strength (R-PE) fluorescence intensity (Figure 4).