A detailed protocol is shipped with every Novex® ELISA kit. The general ELISA protocol, however, is quite simple and is shown in Figure 10 in schematic form. The procedure for Chemi ELISA kits is similar, except the readout is chemiluminescent instead of colorimetric.
The assay procedure for phosphoELISA™ kits is similar to our general ELISA procedure with only a few minor differences (Figure 1). The detection antibody for ELISA kits is biotin-labeled and is followed by incubation with streptavidin-HRP, whereas the detection antibody for phosphoELISA™ kits is a rabbit polyclonal antibody and is followed by incubation with HRP-labeled anti–rabbit IgG.
|Figure 1||Figure 2