Two-parameter cell health assay
This kit enables simultaneous determination of live and dead cells with two probes: CellTrace™ calcein violet indicates cell viability based on plasma membrane integrity, and aqua-fluorescent reactive dye measures cell vitality based on intracellular esterase activity.
This protocol can be used for:
- Identifying live and dead cells using a flow cytometer
This protocol should not be used for:
- Fluorescence microscopy
You will need the following for this protocol:
- Cells growing in culture
- LIVE/DEAD® Violet Viability/Vitality Kit (Cat. No. L34958)
- Flow cytometer
Protocol
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Protocol tips
- Cell concentration should be 1 x 105 to 5 x 106 cells per mL
- Stock solutions of both dyes should preferably be used within 24 hours
- Calcein violet AM may hydrolyze if exposed to moisture
- Not compatible with fixation
Staining pattern of a mixture of heat-killed and untreated Jurkat cells (human leukemia T-cell) stained with the LIVE/DEAD® Violet Viability/Vitality Kit.
Spectral information and storage
Aqua-fluorescent reactive dye | Calcein violet AM | |
---|---|---|
Excitation/Emission (in nm) | 367/526 | 400/452 |
Storage conditions | –20°C | –20°C |