NucGreen Dead 488 ReadyProbes Reagent is a cell-impermeant stain that emits bright green fluorescence when bound to DNA. Since this reagent stains cells that lack plasma membrane integrity, it is extremely useful to measure viability and to measure cytotoxicity in kinetic live-cell assays. NucGreen Dead 488 reagent is also suitable for staining nuclei in fixed-cell preparations and tissue sections.
|1. Culture cells in an appropriate medium and vessel for fluorescence microscopy
|2. Add 2 drops NucGreen Dead 488 reagent per milliliter of medium
|3. Incubate for 5 minutes at 20–25°C
|4. Image the cells
|NucGreen Dead 488
|Standard filter set
|FITC or GFP
|EVOS Light Cube
HeLa cells stained with NucGreen Dead 488 ReadyProbes reagent, indicating loss of plasma membrane integrity.