Product Description

Supplement S7 is a filter-sterilized, concentrated (100X), ionically balanced solution intended for use as one
component in a complete culture environment for normal human epidermal keratinocytes. Supplement S7 is chemically defined and animal origin-free (AOF).  Each 5 ml bottle of Supplement S7 is the correct amount
of supplement for a 500 ml bottle of EpiLife® basal medium.

Intended Use

Supplement S7 is intended for use in conjunction with EpiLife® basal medium for the routine defined, animal
origin-free culture of normal human epidermal keratinocytes. Additional applications for this product may include primary isolation of keratinocytes from skin. Supplement S7 must be used in conjunction with Coating Matrix Kit (Cat. R-011-K).

This product is for research use only. Not intended for human or animal therapeutic or diagnostic use.

Caution: If handled improperly, some components of this product may present a health hazard. Take
appropriate precautions when handling this product, including the wearing of protective clothing and eyewear. Dispose of properly.

Storage and Stability

Supplement S7 is stored at –20°C at our facility and is shipped on dry ice. Upon receipt, the product should be
stored at –20°C in a freezer that is not self-defrosting. When stored at –20°C the product is stable until the
expiration date shown on the label.


To thaw, place the product in a 37° C water bath or overnight at 4° C. If thawed in a water bath, do not leave
the product at 37° C after the product has thawed. For instructions on adding Supplement S7 to EpiLife® basal medium, please refer to the instructions that accompany the basal medium.

S-017-5               2009