protocol

Our Lipofectamine Reagent Protocols have been optimized for efficiency, viability, and reproducibility across a broad range of cell types. This is often the best place to start, especially in a new cell line. If you find this doesn’t work for your specific cell type, then you can you look to our cell specific protocols for further optimization.


Transfection protocols

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Cell type Transfected nucleic acid Transfection reagent
A549 human lung epithelial carcinoma cells plasmid DNA Lipofectamine 3000
A549 human lung epithelial carcinoma cells plasmid DNA Lipofectamine LTX
ACHN human renal adenocarcinoma cells plasmid DNA Lipofectamine LTX
C6 rat glioma cells plasmid DNA Lipofectamine LTX
Caco-2 human adenocarcinoma cells plasmid DNA Lipofectamine LTX
CHO-K1 cells plasmid DNA Lipofectamine LTX
COS-7 monkey kidney fibroblasts plasmid DNA Lipofectamine LTX
DU 145 human prostate epithelial carcinoma cells plasmid DNA Lipofectamine 3000
eukaryotic cells DNA, RNA DMRIE-C
eukaryotic cells plasmid DNA FreeStyle MAX
eukaryotic cells DNA Lipofectamine
eukaryotic cells DNA, RNA Lipofectamine 2000
eukaryotic cells DNA Lipofectamine 2000 CD
eukaryotic cells DNA Lipofectamine LTX and PLUS
eukaryotic cells siRNA, oligos Oligofectamine
eukaryotic cells pre-transfection DNA complexes PLUS pre-transfection enhancer
FreeStyle 293-F cells plasmid DNA 293fectin
GripTite 293 MSR HECs plasmid DNA Lipofectamine LTX
HCT 116 human colorectal carcinoma cells plasmid DNA Lipofectamine LTX
HEK293 cells plasmid DNA Lipofectamine LTX
HEK293 suspension cells plasmid DNA 293fectin
HeLa S3 human cervical adenocarcinoma cells plasmid DNA Lipofectamine LTX
Hep 3B2.1-7 human hepatocellular carcinoma cells plasmid DNA Lipofectamine 3000
Hep G2 human hepatocellular carcinoma cells plasmid DNA Lipofectamine 3000
Hep G2 human hepatocellular carcinoma cells plasmid DNA Lipofectamine LTX
Hepa 1-6 mouse hepatoma cells plasmid DNA Lipofectamine 3000
HL-60 human lymphoblast cells plasmid DNA Lipofectamine LTX
Hs 578T human mammary epithelial carcinoma cells plasmid DNA Lipofectamine 3000
HT 29 human colorectal adenocarcinoma cells plasmid DNA Lipofectamine LTX
HT-1080 human fibrosarcoma cells plasmid DNA Lipofectamine LTX
HUVEC plasmid DNA Lipofectamine LTX
HUVEC siRNA, Stealth RNAi RNAiMAX
IMR-90 human embryonic lung cells plasmid DNA Lipofectamine LTX
Jurkat human T cell leukemia cells plasmid DNA Lipofectamine LTX
K-562 human myelogenous leukemia cells plasmid DNA Lipofectamine LTX
LNCaP human prostate epithelial carcinoma cells plasmid DNA Lipofectamine 3000
LNCaP human prostate epithelial carcinoma cells plasmid DNA Lipofectamine LTX
mammalian cells dsRNA oligo (BLOCK-iT fluorescent oligo) cationic lipid
mammalian cells siRNA, Stealth RNAi + plasmid DNA (co-transfection) Lipofectamine 2000
mammalian cells siRNA, Stealth RNAi Lipofectamine 2000
mammalian cells DNA, RNA, oligos Lipofectin
mammalian cells siRNA, Stealth RNAi RNAiMAX
mammalian cells, reverse transfection siRNA, Stealth RNAi Lipofectamine 2000
mammalian cells, reverse transfection siRNA, Stealth RNAi RNAiMAX
MCF 10A human mammary epithelial cells plasmid DNA Lipofectamine 3000
MCF7 human breast adenocarcinoma cells plasmid DNA Lipofectamine 3000
MCF7 human breast adenocarcinoma cells plasmid DNA Lipofectamine LTX
MDA-MB-231 human breast adenocarcinoma cells plasmid DNA Lipofectamine 3000
MDA-MB-231 human breast adenocarcinoma cells plasmid DNA Lipofectamine LTX
MDA-MB-435 cells plasmid DNA Lipofectamine LTX
MDA-MB-453 human breast adenocarcinoma cells plasmid DNA Lipofectamine 3000
MDA-MB-468 human breast adenocarcinoma cells plasmid DNA Lipofectamine 3000
MOLT-4 human T cell lymphoblast cells plasmid DNA Lipofectamine LTX
NCI-H23 non-small cell lung cancer cells plasmid DNA Lipofectamine 3000
NCI-H460 human large cell lung cancer cells plasmid DNA Lipofectamine 3000
NCI-H460 human large cell lung cancer cells plasmid DNA Lipofectamine LTX
NIH/3T3 mouse embryonic fibroblasts plasmid DNA Lipofectamine LTX
Normal human foreskin fibroblasts (NHFF) plasmid DNA Lipofectamine LTX
NTERA-2 human embryonal carcinoma stem cells plasmid DNA Lipofectamine LTX
PC-12 rat neuronal cells plasmid DNA Lipofectamine LTX
PC-3 human prostate adenocarcinoma cells plasmid DNA Lipofectamine LTX
plant protoplasts DNA Lipofectin
Primary mouse neural progenitor cells (mNPC) plasmid DNA Lipofectamine LTX
RAW 264.7 mouse macrophage cells plasmid DNA Lipofectamine LTX
Sf9, Sf21 baculovirus DNA Cellfectin II
SK-BR-3 human breast adenocarcinoma cells plasmid DNA Lipofectamine 3000
SK-BR-3 human breast adenocarcinoma cells plasmid DNA Lipofectamine LTX
SK-MEL-28 human malignant melanoma cells plasmid DNA Lipofectamine LTX
SK-OV-3 human ovarian adenocarcinoma cells plasmid DNA Lipofectamine LTX
stem cells, (hMSCs) siRNA, Stealth RNAi Lipofectamine 2000
stem cells, (hPSCs) in Essential 8 Medium plasmid DNA, mRNA, RNP Lipofectamine Stem
stem cells, (hPSCs) in mTeSR1 Medium on Geltrex matrix plasmid DNA, mRNA, RNP Lipofectamine Stem
stem cells, (hPSCs) in StemFlex Medium plasmid DNA, mRNA, RNP Lipofectamine Stem
stem cells, neural plasmid DNA, mRNA, RNP Lipofectamine Stem
THP-1 human monocytic leukemia cells plasmid DNA Lipofectamine LTX