protocol

Our Lipofectamine Reagent Protocols have been optimized for efficiency, viability, and reproducibility across a broad range of cell types. This is often the best place to start, especially in a new cell line. If you find this doesn’t work for your specific cell type, then you can you look to our cell specific protocols for further optimization.


Transfection protocols

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Cell typeTransfected nucleic acidTransfection reagent
A549 human lung epithelial carcinoma cellsplasmid DNALipofectamine 3000
A549 human lung epithelial carcinoma cellsplasmid DNALipofectamine LTX
ACHN human renal adenocarcinoma cellsplasmid DNALipofectamine LTX
C6 rat glioma cellsplasmid DNALipofectamine LTX
Caco-2 human adenocarcinoma cellsplasmid DNALipofectamine LTX
CHO-K1 cellsplasmid DNALipofectamine LTX
COS-7 monkey kidney fibroblastsplasmid DNALipofectamine LTX
DU 145 human prostate epithelial carcinoma cellsplasmid DNALipofectamine 3000
eukaryotic cellsDNA, RNADMRIE-C
eukaryotic cellsplasmid DNAFreeStyle MAX
eukaryotic cellsDNALipofectamine
eukaryotic cellsDNA, RNALipofectamine 2000
eukaryotic cellsDNALipofectamine 2000 CD
eukaryotic cellsDNALipofectamine LTX and PLUS
eukaryotic cellssiRNA, oligosOligofectamine
eukaryotic cellspre-transfection DNA complexesPLUS pre-transfection enhancer
FreeStyle 293-F cellsplasmid DNA293fectin
GripTite 293 MSR HECsplasmid DNALipofectamine LTX
HCT 116 human colorectal carcinoma cellsplasmid DNALipofectamine LTX
HEK293 cellsplasmid DNALipofectamine LTX
HEK293 suspension cellsplasmid DNA293fectin
HeLa S3 human cervical adenocarcinoma cellsplasmid DNALipofectamine LTX
Hep 3B2.1-7 human hepatocellular carcinoma cellsplasmid DNALipofectamine 3000
Hep G2 human hepatocellular carcinoma cellsplasmid DNALipofectamine 3000
Hep G2 human hepatocellular carcinoma cellsplasmid DNALipofectamine LTX
Hepa 1-6 mouse hepatoma cellsplasmid DNALipofectamine 3000
HL-60 human lymphoblast cellsplasmid DNALipofectamine LTX
Hs 578T human mammary epithelial carcinoma cellsplasmid DNALipofectamine 3000
HT 29 human colorectal adenocarcinoma cellsplasmid DNALipofectamine LTX
HT-1080 human fibrosarcoma cellsplasmid DNALipofectamine LTX
HUVECplasmid DNALipofectamine LTX
HUVECsiRNA, Stealth RNAiRNAiMAX
IMR-90 human embryonic lung cellsplasmid DNALipofectamine LTX
Jurkat human T cell leukemia cellsplasmid DNALipofectamine LTX
K-562 human myelogenous leukemia cellsplasmid DNALipofectamine LTX
LNCaP human prostate epithelial carcinoma cellsplasmid DNALipofectamine 3000
LNCaP human prostate epithelial carcinoma cellsplasmid DNALipofectamine LTX
mammalian cellsdsRNA oligo (BLOCK-iT fluorescent oligo)cationic lipid
mammalian cellssiRNA, Stealth RNAi + plasmid DNA (co-transfection)Lipofectamine 2000
mammalian cellssiRNA, Stealth RNAiLipofectamine 2000
mammalian cellsDNA, RNA, oligosLipofectin
mammalian cellssiRNA, Stealth RNAiRNAiMAX
mammalian cells, reverse transfectionsiRNA, Stealth RNAiLipofectamine 2000
mammalian cells, reverse transfectionsiRNA, Stealth RNAiRNAiMAX
MCF 10A human mammary epithelial cellsplasmid DNALipofectamine 3000
MCF7 human breast adenocarcinoma cellsplasmid DNALipofectamine 3000
MCF7 human breast adenocarcinoma cellsplasmid DNALipofectamine LTX
MDA-MB-231 human breast adenocarcinoma cellsplasmid DNALipofectamine 3000
MDA-MB-231 human breast adenocarcinoma cellsplasmid DNALipofectamine LTX
MDA-MB-435 cellsplasmid DNALipofectamine LTX
MDA-MB-453 human breast adenocarcinoma cellsplasmid DNALipofectamine 3000
MDA-MB-468 human breast adenocarcinoma cellsplasmid DNALipofectamine 3000
MOLT-4 human T cell lymphoblast cellsplasmid DNALipofectamine LTX
NCI-H23 non-small cell lung cancer cellsplasmid DNALipofectamine 3000
NCI-H460 human large cell lung cancer cellsplasmid DNALipofectamine 3000
NCI-H460 human large cell lung cancer cellsplasmid DNALipofectamine LTX
NIH/3T3 mouse embryonic fibroblastsplasmid DNALipofectamine LTX
Normal human foreskin fibroblasts (NHFF)plasmid DNALipofectamine LTX
NTERA-2 human embryonal carcinoma stem cellsplasmid DNALipofectamine LTX
PC-12 rat neuronal cellsplasmid DNALipofectamine LTX
PC-3 human prostate adenocarcinoma cellsplasmid DNALipofectamine LTX
plant protoplastsDNALipofectin
Primary mouse neural progenitor cells (mNPC)plasmid DNALipofectamine LTX
RAW 264.7 mouse macrophage cellsplasmid DNALipofectamine LTX
Sf9, Sf21baculovirus DNACellfectin II
SK-BR-3 human breast adenocarcinoma cellsplasmid DNALipofectamine 3000
SK-BR-3 human breast adenocarcinoma cellsplasmid DNALipofectamine LTX
SK-MEL-28 human malignant melanoma cellsplasmid DNALipofectamine LTX
SK-OV-3 human ovarian adenocarcinoma cellsplasmid DNALipofectamine LTX
stem cells, (hMSCs)siRNA, Stealth RNAiLipofectamine 2000
stem cells, (hPSCs) in Essential 8 Mediumplasmid DNA, mRNA, RNPLipofectamine Stem
stem cells, (hPSCs) in mTeSR1 Medium on Geltrex matrixplasmid DNA, mRNA, RNPLipofectamine Stem
stem cells, (hPSCs) in StemFlex Mediumplasmid DNA, mRNA, RNPLipofectamine Stem
stem cells, neuralplasmid DNA, mRNA, RNPLipofectamine Stem
THP-1 human monocytic leukemia cellsplasmid DNALipofectamine LTX