The Invitrogen Novex SeeBlue Plus2 Pre-Stained Standard allows easy visualization of protein molecular weight ranges during electrophoresis and evaluation of western transfer efficiency. The important features of the standard are listed below:
- Consists of 10 pre-stained protein bands (8 blue and 2 contrasting colors) in the range of 4-250 kDa
- Suitable for Tris-Glycine, Tricine, and Invitrogen NuPAGE Novex Gels
The SeeBlue Plus2 Pre-Stained Standard is supplied ready-to-use. There is no need to heat or add reducing agent.
Use loading volumes listed below to obtain the best results:
|Mini-Gel (1.0 mm thick)||10 µl||3 µl|
|Mini-Gel (1.5 mm thick)||20 µl||5 µl|
|Standard Gel (1.0 mm thick)||20 µl||7 µl|
|Standard Gel (1.5 mm thick)||30 µl||10 µl|
The apparent molecular weights of the protein bands in SeeBlue Plus2 Pre-Stained Standard in several buffer systems is shown below. The protein bands have different mobilities in various SDS-PAGE buffer systems.
Contents: 500 µl of SeeBlue Plus2 Pre-Stained Standard
Loading Buffer: Tris-HCl, Formamide, SDS, Phenol Red
Storage: Store at 4°C
Stability: 4 months at 4°C