Organism Type Primate - Non Human A COS-like Monkey kidney cell line, CMT3, was transfected by the standard CaPO4 method with plasmids pCMVgagpolrre-r and pCMVrev and a selective marker plasmid that expresses the hygromycin resistance gene to generate cell line B4.14. The pCMVrev gene is required for efficient expression of the gag gene. The cells were maintained in Iscove’s modified Dulbecco’s medium with 10% fetal calf serum, 50 mg of gentamicin per ml, and 200 mg of hygromycin B per ml.
Tissues Kidney
Phenotype Adherent
Primary yes
Application Transient Transfection

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