Organism Type Hamster Previous results showed that when apoB53 is stably expressed in CHO cells (B53 cells), it is rapidly degraded, producing an N-terminal fragment that is secreted without a lipid core . The proteolytic inhibitor ALLN blocks the formation of the N-terminal apoB fragment and causes intact apoB53 to accumulate as a transmembrane protein in isolated microsomes. These cells, stably transfected with a plasmid expressing rat 7-hydroxylase, are hereafter referred to as JF7 cells.
Tissues Ovary
Phenotype Adherent
Primary yes
Application

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