ctll-2 Cells | Thermo Fisher Scientific

Organism Type	Mouse	Cytotoxic T cell line (CD8+) Subculturing: Subculture actively growing suspension cultures before they the reached 1 X 10 exp5 cell/ml. Use inoculation densities of 100 to 1000 cells/ml. Do not allow cells to exceed 1 X 10 exp5 cells/ml or IL-2 will be rapidly depleted and the cells will quickly lose viability. Split ratio: Subcultivation ratios of 1:100 to 1:1000 are recommended
Tissues	Lymphocyte
Phenotype	Suspension
Primary	no
Application	Cell Culture / Growth Conditions

Every step of the way, a wide range of cell health products

Maintaining healthy cells is the key to experimental success and reproducible research results. To give you confidence in the health of your cells every step of the way, we’ve highlighted the technologies and products within cell biology that are critical to maintaining optimal cell health. No matter how you are using your cells, you can count on these products to help keep them healthy.

GROW Gibco cell culture media & reagents Gibco growth factors Gibco FBS Countess II FL Automated Cell Counter		MODIFY Lipofectamine transfection reagents Neon electroporation transfection system Invitrogen RNAi products Recombinant proteins Dynabeads cell separation technology		DETECT Invitrogen imaging reagents Invitrogen flow cytometry reagents		ANALYZE EVOS cell imaging systems Attune NxT Flow Cytometer Qubit fluorometric quantification