Organism Type Rat Kirsten Murine Sarcoma Virus transformed rat kidney epithelial cells. Cultures can be maintained by transferring floating cells to additional flasks. Attached cells may be subcultured using 0.25% trypsin-0.03% EDTA. Remove medium, add fresh 0.25% trypsin solution for 2 to 3 minutes, remove trypsin and let the culture sit at 37C for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks.
Tissues Epithelial
Phenotype Either
Primary no
Application Cell Culture / Growth Conditions,Stable Cell Transfection

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