Organism Type Rabbit Male New Zealand rabbits (1-2 kg) were anesthetized with 30 mg/kg pentobarbital (Abbott Laboratories, North Chicago, IL), and the thorax and abdomen were opened by a midline incision. The aorta was rapidly removed, and VSMC were isolated. Cells between four and eight passages were plated in 12 or 24 wells or 100-mm plates. Cells were maintained under 5% CO2 in M-199 medium with penicillin, streptomycin, and 10% FBS.
Tissues Aorta
Phenotype Adherent
Primary yes
Application Stable Cell Transfection,Transient Transfection

Every Step of the Way, a Wide Range of Cell Health Products

Maintaining healthy cells is the key to experimental success and reproducible research results. To give you confidence in the health of your cells every step of the way, we’ve highlighted the technologies and products within cell biology that are critical to maintaining optimal cell health. No matter how you are using your cells, you can count on these products to help keep them healthy.