YM Medium, powder
INTENDED USE: Growth and expression of Agrobacterium. YM Medium improves transformation efficiency for electroporation of Agrobacterium by up to 40-fold when compared with LB broth. Also, no aggregation of Agrobacterium was observed when YM Medium was used in culturing the bacteria for plant infection. INSTRUCTIONS FOR USE: Suspend contents of 1 packet in 1 L of demineralized water. Autoclave for 15 min at 121°C. To prepare YM agar: Suspend contents of 1 packet in 1 L of demineralized water. Add 15 g of SELECT Agar and boil while stirring to dissolve all ingredients completely. Autoclave for 15 min at 121°C. Cool down to 50C before adding antibiotics (optional) and pouring into plates. RECOMMENDED STORAGE CONDITION: 15°C TO 30°C.
|Components||Molecular Weight||Concentration (mg/L)||mM|
|Dipotassium hydrogen phosphate||0.5||Infinity|
|Magnesium Sulfate (MgSO4-7H2O)||0.2||Infinity|
|Sodium Chloride (NaCl)||0.1||Infinity|
|SELECT Yeast Extract||0.4||Infinity|
1. Lin J.J. (1994) Focus® 16, 18.
2. Lin J.J. (1994) Plant Science 10, 11.
3. Lin J.J. Assad-Garcia, N. and Kuo, J. (1994) Focus® 16, 72.
Cat No: 22711022
Cat No: 22712020
Gibco® BG-11 Media: Optimized for Cyanobacteria
Cat No: a1379901 , A1379902
TAP Growth Media, optimized for Chlamydomonas culture
Cat No: A1379801 , A1379802