Technical Resources

YM Medium, powder

INTENDED USE: Growth and expression of Agrobacterium. YM Medium improves transformation efficiency for electroporation of Agrobacterium by up to 40-fold when compared with LB broth. Also, no aggregation of Agrobacterium was observed when YM Medium was used in culturing the bacteria for plant infection. INSTRUCTIONS FOR USE: Suspend contents of 1 packet in 1 L of demineralized water. Autoclave for 15 min at 121°C. To prepare YM agar: Suspend contents of 1 packet in 1 L of demineralized water. Add 15 g of SELECT Agar and boil while stirring to dissolve all ingredients completely. Autoclave for 15 min at 121°C. Cool down to 50C before adding antibiotics (optional) and pouring into plates. RECOMMENDED STORAGE CONDITION: 15°C TO 30°C.

  • Catalog Number(s)

ComponentsMolecular WeightConcentration (mg/L)mM
Inorganic Salts
Dipotassium hydrogen phosphate0.5Infinity
Magnesium Sulfate (MgSO4-7H2O)0.2Infinity
Sodium Chloride (NaCl)0.1Infinity
Other Components
SELECT Yeast Extract0.4Infinity


1. Lin J.J. (1994) Focus® 16, 18.

2. Lin J.J. (1994) Plant Science 10, 11.

3. Lin J.J. Assad-Garcia, N. and Kuo, J. (1994) Focus® 16, 72.

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