Technical Resources - Media Formulations

41010 - Basal Medium Eagle (BME), no Glutamine

Components	Molecular Weight	Concentration (mg/L)	mM
Amino Acids
L-Arginine hydrochloride	211.0	21.0	0.09952607
L-Cystine	240.0	12.0	0.05
L-Histidine	155.0	8.0	0.051612902
L-Isoleucine	131.0	26.0	0.19847329
L-Leucine	131.0	26.0	0.19847329
L-Lysine hydrochloride	183.0	29.0	0.15846995
L-Methionine	149.0	7.5	0.05033557
L-Phenylalanine	165.0	16.5	0.1
L-Threonine	119.0	24.0	0.20168068
L-Tryptophan	204.0	4.0	0.019607844
L-Tyrosine	181.0	18.0	0.09944751
L-Valine	117.0	23.5	0.2008547
Vitamins
Biotin	244.0	1.0	0.0040983604
Choline chloride	140.0	1.0	0.007142857
D-Calcium pantothenate	477.0	1.0	0.002096436
Folic Acid	441.0	1.0	0.0022675737
Nicotinamide	122.0	1.0	0.008196721
Pyridoxal hydrochloride	204.0	1.0	0.004901961
Riboflavin	376.0	0.1	2.6595744E-4
Thiamine hydrochloride	337.0	1.0	0.002967359
i-Inositol	180.0	1.8	0.01
Inorganic Salts
Calcium Chloride (CaCl2-2H2O)	147.0	264.0	1.7959183
Magnesium Sulfate (MgSO4-7H2O)	246.0	200.0	0.8130081
Potassium Chloride (KCl)	75.0	400.0	5.3333335
Sodium Bicarbonate (NaHCO3)	84.0	2200.0	26.190475
Sodium Chloride (NaCl)	58.0	6800.0	117.24138
Sodium Phosphate monobasic (NaH2PO4-2H2O)	156.0	158.0	1.0128205
Other Components
D-Glucose (Dextrose)	180.0	1000.0	5.5555553
Phenol Red	376.4	10.0	0.026567481

Referencia

1. Eagle, H. (1965) Proc. Exp. Biol. Med., 89:362.