Technical Resources

41550 - Ham's F-10 Nutrient Mix, GlutaMAX(TM)

  • Catalog Number(s)

ComponentsMolecular WeightConcentration (mg/L)mM
Amino Acids
Glycine75.07.50.1
L-Alanine89.09.00.101123594
L-Alanyl-L-Glutamine217.0217.01.0
L-Arginine hydrochloride211.0211.01.0
L-Asparagine-H2O150.015.00.1
L-Aspartic acid133.013.00.09774436
L-Cysteine121.025.00.20661157
L-Glutamic Acid147.014.70.1
L-Histidine hydrochloride-H2O210.023.00.10952381
L-Isoleucine131.02.60.019847328
L-Leucine131.013.00.099236645
L-Lysine hydrochloride183.029.00.15846995
L-Methionine149.04.50.030201342
L-Phenylalanine165.05.00.030303031
L-Proline115.011.50.1
L-Serine105.010.50.1
L-Threonine119.03.60.0302521
L-Tryptophan204.00.60.0029411765
L-Tyrosine181.01.80.009944751
L-Valine117.03.50.02991453
Vitamins
Biotin244.00.0249.836066E-5
Choline chloride140.00.70.005
D-Calcium pantothenate477.00.70.0014675052
Folic Acid441.01.30.0029478457
Niacinamide122.00.60.004918033
Pyridoxine hydrochloride206.00.29.708738E-4
Riboflavin376.00.40.0010638298
Thiamine hydrochloride337.01.00.002967359
Vitamin B121355.01.40.0010332103
i-Inositol180.00.50.0027777778
Inorganic Salts
Calcium Chloride (CaCl2-2H2O)147.044.00.2993197
Cupric sulfate (CuSO4-5H2O)250.00.00251.0E-5
Ferric sulfate (FeSO4-7H2O)278.00.8340.003
Magnesium Sulfate (MgSO4-7H2O)246.0153.00.6219512
Potassium Chloride (KCl)75.0285.03.8
Potassium Phosphate monobasic (KH2PO4)136.083.00.6102941
Sodium Bicarbonate (NaHCO3)84.01200.014.285714
Sodium Chloride (NaCl)58.07400.0127.586205
Sodium Phosphate dibasic (Na2HPO4) anhydrous142.0154.51.0880282
Zinc sulfate (ZnSO4-7H2O)288.00.031.0416666E-4
Other Components
D-Glucose (Dextrose)180.01100.06.111111
Hypoxanthine136.04.00.029411765
Lipoic Acid206.00.29.708738E-4
Phenol Red376.41.20.0031880978
Sodium Pyruvate110.0110.01.0
Thymidine242.00.70.002892562

Reference:

1. Ham, R.G., (1963) An improved nutrient solution for diploid Chinese hamster and human cell lines. Exp. Cell Res., 29:515.

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