Technical Resources - Media Formulations

A10489 - MEM, Richter’s, no Gentamycin

Components	Molecular Weight	Concentration (mg/L)	mM
Amino Acids
L-Arginine	174.0	127.0	0.72988504
L-Asparagine	132.0	60.0	0.45454547
L-Cystine	240.0	24.0	0.1
L-Glutamine	146.0	292.0	2.0
L-Histidine hydrochloride-H2O	210.0	42.0	0.2
L-Isoleucine	131.0	52.0	0.39694658
L-Leucine	131.0	131.0	1.0
L-Lysine hydrochloride	183.0	73.0	0.3989071
L-Methionine	149.0	15.0	0.10067114
L-Phenylalanine	165.0	32.0	0.19393939
L-Serine	105.0	42.0	0.4
L-Threonine	119.0	48.0	0.40336135
L-Tryptophan	204.0	10.0	0.04901961
L-Tyrosine	181.0	36.0	0.19889502
L-Valine	117.0	46.0	0.3931624
Vitamins
Biotin	244.0	0.1	4.0983607E-4
Choline chloride	140.0	56.0	0.4
D-Calcium pantothenate	477.0	1.0	0.002096436
Folic Acid	441.0	2.2	0.0049886624
Niacinamide	122.0	1.0	0.008196721
Pyridoxal hydrochloride	204.0	1.0	0.004901961
Riboflavin	376.0	0.1	2.6595744E-4
Thiamine hydrochloride	337.0	1.0	0.002967359
Vitamin B12	1355.0	1.36	0.0010036901
i-Inositol	180.0	36.0	0.2
Inorganic Salts
Calcium Chloride (CaCl2-2H2O)	147.0	265.0	1.8027211
Ferric Chloride 6H2O	270.0	0.54	0.002
Magnesium Chloride (MgCl2-6H2O)	203.0	183.0	0.9014778
Magnesium Sulfate (MgSO4-7H2O)	246.0	25.0	0.101626016
Potassium Chloride (KCl)	75.0	400.0	5.3333335
Sodium Bicarbonate (NaHCO3)	84.0	2200.0	26.190475
Sodium Chloride (NaCl)	58.0	6800.0	117.24138
Sodium Phosphate monobasic (NaH2PO4-H2O)	138.0	150.0	1.0869565
Zinc sulfate (ZnSO4-7H2O)	288.0	0.14	4.8611112E-4
Other Components
D-Glucose (Dextrose)	180.0	2000.0	11.111111
Linoleic Acid	280.0	0.084	2.9999999E-4
Lipoic Acid	206.0	0.2	9.708738E-4
Phenol Red	376.4	10.0	0.026567481
Putrescine 2HCl	161.0	0.16	9.937888E-4
Sodium Pyruvate	110.0	110.0	1.0

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