Technical Resources

21157 - Medium 199, 2x, no phenol red

  • Catalog Number(s)

Components Molecular Weight Concentration (mg/L) mM
Amino Acids
Glycine 75.0 100.0 1.3333334
L-Alanine 89.0 50.0 0.56179774
L-Arginine hydrochloride 211.0 140.0 0.6635071
L-Aspartic acid 133.0 60.0 0.45112783
L-Cysteine hydrochloride-H2O 176.0 0.2 0.0011363636
L-Cystine 2HCl 240.0 52.0 0.21666667
L-Glutamic Acid 147.0 150.0 1.0204082
L-Glutamine 146.0 200.0 1.369863
L-Histidine hydrochloride-H2O 210.0 53.76 0.25599998
L-Hydroxyproline 131.0 20.0 0.15267175
L-Isoleucine 131.0 80.0 0.610687
L-Leucine 131.0 120.0 0.9160305
L-Lysine hydrochloride 183.0 140.0 0.76502734
L-Methionine 149.0 30.0 0.20134228
L-Phenylalanine 165.0 50.0 0.3030303
L-Proline 115.0 80.0 0.6956522
L-Serine 105.0 50.0 0.47619048
L-Threonine 119.0 60.0 0.5042017
L-Tryptophan 204.0 20.0 0.09803922
L-Tyrosine disodium salt dihydrate 261.0 116.0 0.44444445
L-Valine 117.0 50.0 0.42735043
Vitamins
Ascorbic Acid 176.0 0.1 5.681818E-4
Biotin 244.0 0.02 8.196721E-5
Choline chloride 140.0 1.0 0.007142857
D-Calcium pantothenate 477.0 0.02 4.192872E-5
Folic Acid 441.0 0.02 4.5351473E-5
Menadione (Vitamin K3) 172.0 0.02 1.16279065E-4
Niacinamide 122.0 0.05 4.0983607E-4
Nicotinic acid (Niacin) 123.0 0.05 4.0650408E-4
Para-Aminobenzoic Acid 137.0 0.1 7.2992704E-4
Pyridoxal hydrochloride 204.0 0.05 2.4509805E-4
Pyridoxine hydrochloride 206.0 0.05 2.4271845E-4
Riboflavin 376.0 0.02 5.319149E-5
Thiamine hydrochloride 337.0 0.02 5.934718E-5
Vitamin A (acetate) 328.0 0.2 6.097561E-4
Vitamin D2 (Calciferol) 397.0 0.2 5.0377834E-4
i-Inositol 180.0 0.1 5.5555557E-4
Inorganic Salts
Calcium Chloride (CaCl2-2H2O) 147.0 400.0 2.7210884
Ferric nitrate (Fe(NO3)-9H2O) 404.0 1.4 0.0034653465
Magnesium Sulfate (MgSO4-7H2O) 246.0 195.34 0.794065
Potassium Chloride (KCl) 75.0 800.0 10.666667
Sodium Bicarbonate (NaHCO3) 84.0 2500.0 29.761906
Sodium Chloride (NaCl) 58.0 13600.0 234.48276
Sodium Phosphate dibasic (Na2HPO4) anhydrous 138.0 316.0 2.289855
Other Components
2-deoxy-D-ribose 134.0 1.0 0.0074626864
Adenine sulfate 404.0 20.0 0.04950495
Adenosine 5'-phosphate 347.0 0.4 0.0011527378
Adenosine 5'-triphosphate 605.0 2.0 0.0033057851
Cholesterol 387.0 0.4 0.0010335917
D-Glucose (Dextrose) 180.0 2000.0 11.111111
Glutathione (reduced) 307.0 0.1 3.257329E-4
Guanine hydrochloride 188.0 0.6 0.0031914895
Hypoxanthine 136.0 0.8 0.005882353
Ribose 150.0 1.0 0.006666667
Sodium acetate-3H2O 136.0 100.0 0.7352941
Thymine 126.0 0.6 0.004761905
Tween 80® 40.0 Infinity
Uracil 112.0 0.6 0.005357143
Xanthine 174.0 0.68 0.003908046

Reference:

1. Morgan, J.F. and Campbell, M.E. (1955) J. Natl. Cancer Inst., 16:557.

2. Morgan, J.F., Morton, H.J. and Parker R.C. (1950) Proc. Soc. Exp. Biol. Med., 73:1.

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