Technical Resources

21157 - Medium 199, 2x, no phenol red

  • Catalog Number(s)

ComponentsMolecular WeightConcentration (mg/L)mM
Amino Acids
Glycine75.0100.01.3333334
L-Alanine89.050.00.56179774
L-Arginine hydrochloride211.0140.00.6635071
L-Aspartic acid133.060.00.45112783
L-Cysteine hydrochloride-H2O176.00.20.0011363636
L-Cystine 2HCl240.052.00.21666667
L-Glutamic Acid147.0150.01.0204082
L-Glutamine146.0200.01.369863
L-Histidine hydrochloride-H2O210.053.760.25599998
L-Hydroxyproline131.020.00.15267175
L-Isoleucine131.080.00.610687
L-Leucine131.0120.00.9160305
L-Lysine hydrochloride183.0140.00.76502734
L-Methionine149.030.00.20134228
L-Phenylalanine165.050.00.3030303
L-Proline115.080.00.6956522
L-Serine105.050.00.47619048
L-Threonine119.060.00.5042017
L-Tryptophan204.020.00.09803922
L-Tyrosine disodium salt dihydrate261.0116.00.44444445
L-Valine117.050.00.42735043
Vitamins
Ascorbic Acid176.00.15.681818E-4
Biotin244.00.028.196721E-5
Choline chloride140.01.00.007142857
D-Calcium pantothenate477.00.024.192872E-5
Folic Acid441.00.024.5351473E-5
Menadione (Vitamin K3)172.00.021.16279065E-4
Niacinamide122.00.054.0983607E-4
Nicotinic acid (Niacin)123.00.054.0650408E-4
Para-Aminobenzoic Acid137.00.17.2992704E-4
Pyridoxal hydrochloride204.00.052.4509805E-4
Pyridoxine hydrochloride206.00.052.4271845E-4
Riboflavin376.00.025.319149E-5
Thiamine hydrochloride337.00.025.934718E-5
Vitamin A (acetate)328.00.26.097561E-4
Vitamin D2 (Calciferol)397.00.25.0377834E-4
i-Inositol180.00.15.5555557E-4
Inorganic Salts
Calcium Chloride (CaCl2-2H2O)147.0400.02.7210884
Ferric nitrate (Fe(NO3)-9H2O)404.01.40.0034653465
Magnesium Sulfate (MgSO4-7H2O)246.0195.340.794065
Potassium Chloride (KCl)75.0800.010.666667
Sodium Bicarbonate (NaHCO3)84.02500.029.761906
Sodium Chloride (NaCl)58.013600.0234.48276
Sodium Phosphate dibasic (Na2HPO4) anhydrous138.0316.02.289855
Other Components
2-deoxy-D-ribose134.01.00.0074626864
Adenine sulfate404.020.00.04950495
Adenosine 5'-phosphate347.00.40.0011527378
Adenosine 5'-triphosphate605.02.00.0033057851
Cholesterol387.00.40.0010335917
D-Glucose (Dextrose)180.02000.011.111111
Glutathione (reduced)307.00.13.257329E-4
Guanine hydrochloride188.00.60.0031914895
Hypoxanthine136.00.80.005882353
Ribose150.01.00.006666667
Sodium acetate-3H2O136.0100.00.7352941
Thymine126.00.60.004761905
Tween 80®40.0Infinity
Uracil112.00.60.005357143
Xanthine174.00.680.003908046

Reference:

1. Morgan, J.F. and Campbell, M.E. (1955) J. Natl. Cancer Inst., 16:557.

2. Morgan, J.F., Morton, H.J. and Parker R.C. (1950) Proc. Soc. Exp. Biol. Med., 73:1.

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