Technical Resources

12591 - BGJb Medium (Fitton-Jackson Modification)

Components Molecular Weight Concentration (mg/L) mM
Amino Acids
Glycine 75.0 800.0 10.666667
L-Alanine 89.0 250.0 2.8089888
L-Arginine 174.0 175.0 1.0057471
L-Aspartic acid 133.0 150.0 1.1278195
L-Cysteine hydrochloride-H2O 176.0 101.0 0.5738636
L-Glutamine 146.0 200.0 1.369863
L-Histidine 155.0 150.0 0.9677419
L-Isoleucine 131.0 30.0 0.22900763
L-Leucine 131.0 50.0 0.3816794
L-Lysine 146.0 240.0 1.6438357
L-Methionine 149.0 50.0 0.33557048
L-Phenylalanine 165.0 50.0 0.3030303
L-Proline 115.0 400.0 3.4782608
L-Serine 105.0 200.0 1.9047619
L-Threonine 119.0 75.0 0.6302521
L-Tryptophan 204.0 40.0 0.19607843
L-Tyrosine disodium salt 225.0 58.0 0.25777778
L-Valine 117.0 65.0 0.5555556
Vitamins
Ascorbic Acid 176.0 50.0 0.2840909
Biotin 244.0 0.2 8.1967213E-4
Choline chloride 140.0 50.0 0.35714287
D-Calcium pantothenate 477.0 0.2 4.1928721E-4
DL-alpha Tocopherol phosphate 702.0 1.0 0.0014245014
Folic Acid 441.0 0.2 4.5351475E-4
Niacinamide 122.0 20.0 0.16393442
Para-Aminobenzoic Acid 137.0 2.0 0.01459854
Pyridoxal phosphate 247.0 0.2 8.097166E-4
Riboflavin 376.0 0.2 5.319149E-4
Thiamine hydrochloride 337.0 4.0 0.011869436
Vitamin B12 1355.0 0.04 2.9520295E-5
i-Inositol 180.0 0.2 0.0011111111
Inorganic Salts
Magnesium Sulfate (MgSO4) (anhyd.) 120.0 98.0 0.81666666
Potassium Chloride (KCl) 75.0 400.0 5.3333335
Potassium Phosphate monobasic (KH2PO4) 136.0 160.0 1.1764706
Sodium Bicarbonate (NaHCO3) 84.0 3500.0 41.666668
Sodium Chloride (NaCl) 58.0 5300.0 91.37931
Sodium Phosphate monobasic (NaH2PO4-H2O) 138.0 90.0 0.65217394
Other Components
Calcium lactate 218.0 555.0 2.5458715
D-Glucose (Dextrose) 180.0 10000.0 55.555557
Phenol Red 376.4 20.0 0.053134963
Sodium Acetate 82.0 50.0 0.6097561

Reference:

1. Biggers, J., Gwatrkin, R.B.L. and Heynes, S., Experimental Cell Research, 25:41-58 (1961).

2. The Unpublished modification of Dr. Sylvia Fitton-Jackson was received from Dr. C.A. Bassett, College of Physicians and Surgeons, Columbia University, N.Y., and Dr. Lawrence Raisz, University of Rochester School of Medicine and Dentistry, Rochester, N.Y.

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