Technical Resources

10373 - MEM, Richter's, no phenol red

Components Molecular Weight Concentration (mg/L) mM
Amino Acids
L-Arginine hydrochloride 211.0 126.0 0.5971564
L-Asparagine-H2O 150.0 60.0 0.4
L-Cystine 240.0 24.0 0.1
L-Glutamine 146.0 584.0 4.0
L-Histidine hydrochloride-H2O 210.0 42.0 0.2
L-Isoleucine 131.0 52.0 0.39694658
L-Leucine 131.0 131.0 1.0
L-Lysine hydrochloride 183.0 73.0 0.3989071
L-Methionine 149.0 15.0 0.10067114
L-Phenylalanine 165.0 32.0 0.19393939
L-Proline 115.0 2.0 0.017391304
L-Serine 105.0 42.0 0.4
L-Threonine 119.0 48.0 0.40336135
L-Tryptophan 204.0 10.0 0.04901961
L-Tyrosine 181.0 36.0 0.19889502
L-Valine 117.0 46.0 0.3931624
Vitamins
Biotin 244.0 0.1 4.0983607E-4
Choline chloride 140.0 56.0 0.4
D-Calcium pantothenate 477.0 1.0 0.002096436
Folinic Acid Calcium salt 512.0 1.0 0.001953125
Niacinamide 122.0 1.0 0.008196721
Pyridoxal hydrochloride 204.0 1.0 0.004901961
Riboflavin 376.0 0.1 2.6595744E-4
Thiamine hydrochloride 337.0 1.0 0.002967359
Vitamin B12 1355.0 1.36 0.0010036901
i-Inositol 180.0 36.0 0.2
Inorganic Salts
Calcium Chloride (CaCl2) (anhyd.) 111.0 200.0 1.8018018
Ferric Chloride 6H2O 270.0 0.54 0.002
Magnesium Chloride (anhydrous) 95.0 85.75 0.9026316
Magnesium Sulfate (MgSO4) (anhyd.) 125.0 12.22 0.09776
Potassium Chloride (KCl) 75.0 400.0 5.3333335
Sodium Bicarbonate (NaHCO3) 84.0 2200.0 26.190475
Sodium Chloride (NaCl) 58.0 6800.0 117.24138
Sodium Phosphate monobasic (NaH2PO4) anhydrous 138.0 150.0 1.0869565
Zinc sulfate (ZnSO4-7H2O) 288.0 0.1 3.4722223E-4
Other Components
D-Glucose (Dextrose) 180.0 2000.0 11.111111
Gentimicin Sulfate 0.05 Infinity
Linoleic Acid 280.0 0.1 3.5714285E-4
Lipoic Acid 206.0 0.2 9.708738E-4
Putrescine 2HCl 161.0 0.16 9.937888E-4
Sodium Pyruvate 110.0 110.0 1.0

Reference:

1. Eagle, H. (1959) Science 130:432.

2. Richter, A., Sanford, K., and Evans, V. (1972), J. National Cancer Inst. 49, 1705.