MEK1 Antibody (13-3500) in WB

Western blot analysis of MEK1 was performed by loading 20ug of the indicated whole cell lysates per well and 5ul of PageRuler Plus Prestained Protein Ladder (Product ## 26619) onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane using the G2 Blotter (Product ## 62288), and blocked with 5% milk in TBST for 1 hour at room temperature. MEK1 was detected at ~43kD using a MEK1 monoclonal antibody (Product ## 13-3500) at a dilution of 1ug/ml in 5% milk in TBST overnight at 4C on a rocking platform. After washing, blots were probed with a goat anti-mouse IgG-HRP secondary antibody (Product ## 31430) at a dilution of 1:10,000 for at least 30 minutes at room temperature. Chemiluminescent detection was performed using SuperSignal West Pico substrate (Product ## 34080).

Western blot analysis of MEK1 was performed by loading 20ug of the indicated whole cell lysates per well and 5ul of PageRuler Plus Prestained Protein Ladder (Product ## 26619) onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane using the G2 Blotter (Product ## 62288), and blocked with 5% milk in TBST for 1 hour at room temperature. MEK1 was detected at ~43kD using a MEK1 monoclonal antibody (Product ## 13-3500) at a dilution of 1ug/ml in 5% milk in TBST overnight at 4C on a rocking platform. After washing, blots were probed with a goat anti-mouse IgG-HRP secondary antibody (Product ## 31430) at a dilution of 1:10,000 for at least 30 minutes at room temperature. Chemiluminescent detection was performed using SuperSignal West Pico substrate (Product ## 34080).

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