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alpha Tubulin Antibody (13-8000) in IF
Immunofluorescence analysis of Alpha-Tubulin was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Alpha-Tubulin Mouse monoclonal Antibody (Product ## 13-8000) at 1:250 dilution in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Flour 488 Rabbit Anti-Mouse IgG Secondary Antibody (Product ## A11059) at a dilution of 1:400 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product ## S36938). F-actin (Panel c: red) was stained with Alexa Fluor 594 Phalloidin (Product ## A12381). Panel d is a merged image showing cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 20X magnification.
