TATA Binding Protein Antibody (MA1-189) in WB

Western blot analysis of TBP was performed by loading 20ug of the indicated whole cell lysates and 5ul of PageRuler Plus Prestained Protein Ladder (Product ## 26619) per well onto a Novex 4-20% Tris-Glycine polyacrylamide gel (Product ## WT4202). Proteins were transferred to a nitrocellulose membrane using the G2 Blotter (Product ## 62288), and blocked with 5% Milk in TBST for 1 hour at room temperature. TATA binding protein was detected at ~36-38kD using a TBP monoclonal antibody (Product ## MA1-189) at a dilution of 1:1000 in 5% Milk in TBST overnight at 4C on a rocking platform, followed by a Goat anti-Mouse IgG (H+L) Superclonal Secondary Antibody, HRP conjugate (Product ## A28177) at a dilution of 1:1000 for at least 30 minutes at room temperature. Chemiluminescent detection was performed using SuperSignal Pico substrate (Product ## 34078) and the myECL Imager (Product ## 62236).

Western blot analysis of TBP was performed by loading 20ug of the indicated whole cell lysates and 5ul of PageRuler Plus Prestained Protein Ladder (Product ## 26619) per well onto a Novex 4-20% Tris-Glycine polyacrylamide gel (Product ## WT4202). Proteins were transferred to a nitrocellulose membrane using the G2 Blotter (Product ## 62288), and blocked with 5% Milk in TBST for 1 hour at room temperature. TATA binding protein was detected at ~36-38kD using a TBP monoclonal antibody (Product ## MA1-189) at a dilution of 1:1000 in 5% Milk in TBST overnight at 4C on a rocking platform, followed by a Goat anti-Mouse IgG (H+L) Superclonal Secondary Antibody, HRP conjugate (Product ## A28177) at a dilution of 1:1000 for at least 30 minutes at room temperature. Chemiluminescent detection was performed using SuperSignal Pico substrate (Product ## 34078) and the myECL Imager (Product ## 62236).

Related Products

Related Images

CD335 (NKp46) Antibody (63335182) in RE Go ›

CD223 (LAG-3) Antibody (56223942) in TM Go ›