CUL-1 Antibody (322400) in WB

Western blot analysis was performed on whole cell, tissue extracts (30 ug lysate) of HEL 92.1.7 (Lane 1), MDA-MB-231 (Lane 2), NTERA-2 (Lane 3), MCF-7 (Lane 4), A549 (Lane 5), Caco-2 (Lane 6), Mouse Testis (Lane 7), Rat Testis (Lane 8) and Mouse pancreas (Lane 9). The blots were probed with Anti- CUL1 Mouse Monoclonal Antibody (Product# 322400, 1-2 ug/ml) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.4 ug/ml, 1:2500 dilution). A ~ 89 kDa band corresponding to CUL1 was observed across cell lines tested. Additionally ~25 kDa band was observed across mouse species. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with Pierce™ Power Blotter System (Product # 22834). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Western blot analysis was performed on whole cell, tissue extracts (30 ug lysate) of HEL 92.1.7 (Lane 1), MDA-MB-231 (Lane 2), NTERA-2 (Lane 3), MCF-7 (Lane 4), A549 (Lane 5), Caco-2 (Lane 6), Mouse Testis (Lane 7), Rat Testis (Lane 8) and Mouse pancreas (Lane 9). The blots were probed with Anti- CUL1 Mouse Monoclonal Antibody (Product# 322400, 1-2 ug/ml) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.4 ug/ml, 1:2500 dilution). A ~ 89 kDa band corresponding to CUL1 was observed across cell lines tested. Additionally ~25 kDa band was observed across mouse species. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with Pierce™ Power Blotter System (Product # 22834). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

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