GPR176 Antibody (PA3046) in WB

Western blot analysis of GPR176 was performed by loading 20ug of Mouse Heart tissue lysate and 5ul of PageRuler Plus Prestained Protein Ladder (Product ## 26619) per well onto a 4-20% Tris-Glycine polyacrylamide gel (Product ## WT4202BX10). Proteins were transferred to a nitrocellulose membrane using the G2 Blotter (Product ## 62288), and blocked with 5% Milk in TBST for 1 hour at room temperature. GPR176 was detected at 57kDa using a GPR97 rabbit polyclonal antibody (Product ## PA3-046) at a dilution of 1:2000 in blocking buffer overnight at 4°C on a rocking platform, followed by a Goat anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product ## A16110) at a dilution of 1:5000 for at least 30 minutes at room temperature. Chemiluminescent detection was performed using SuperSignal West Pico (Product ## 34078).

Western blot analysis of GPR176 was performed by loading 20ug of Mouse Heart tissue lysate and 5ul of PageRuler Plus Prestained Protein Ladder (Product ## 26619) per well onto a 4-20% Tris-Glycine polyacrylamide gel (Product ## WT4202BX10). Proteins were transferred to a nitrocellulose membrane using the G2 Blotter (Product ## 62288), and blocked with 5% Milk in TBST for 1 hour at room temperature. GPR176 was detected at 57kDa using a GPR97 rabbit polyclonal antibody (Product ## PA3-046) at a dilution of 1:2000 in blocking buffer overnight at 4°C on a rocking platform, followed by a Goat anti-Rabbit IgG Secondary Antibody, HRP conjugate (Product ## A16110) at a dilution of 1:5000 for at least 30 minutes at room temperature. Chemiluminescent detection was performed using SuperSignal West Pico (Product ## 34078).

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