Occludin Antibody (331594) in WB

Western blot analysis of Occludin was performed by loading 10 ug of CaCO2 lysate per well onto a 4-12% Bis-Tris polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with an Occludin purified monoclonal antibody (Product ## 331500) at a dilution of 1 ug/ml, 0.5 ug/ml, and 0.25 ug/ml for 1 hour at room temperature on a rocking platform and washed in TBS-0.1% Tween-20. Goat Anti-Mouse IGG (H+L) HRP Conjugated secondary antibody (Product## 31430) was used at a concentration of 1:20,000 and incubated for 30 minutes on a rocking platform. Super Signal West Dura substrate (Product ##34076) was incubated for 5 minutes on a rocking platform. Detection was performed using the GBox. The 5th isoform of Occludin was detected at ~23 kDa.

Western blot analysis of Occludin was performed by loading 10 ug of CaCO2 lysate per well onto a 4-12% Bis-Tris polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with an Occludin purified monoclonal antibody (Product ## 331500) at a dilution of 1 ug/ml, 0.5 ug/ml, and 0.25 ug/ml for 1 hour at room temperature on a rocking platform and washed in TBS-0.1% Tween-20. Goat Anti-Mouse IGG (H+L) HRP Conjugated secondary antibody (Product## 31430) was used at a concentration of 1:20,000 and incubated for 30 minutes on a rocking platform. Super Signal West Dura substrate (Product ##34076) was incubated for 5 minutes on a rocking platform. Detection was performed using the GBox. The 5th isoform of Occludin was detected at ~23 kDa.

Related Products

Related Images

CD335 (NKp46) Antibody (63335182) in RE Go ›

CD223 (LAG-3) Antibody (56223942) in TM Go ›