Human IgM Antibody (MA5-14729) in Flow

Flow cytometry analysis of cytoplasmic IgM expression was performed on Ramos (surface and cytoplasmic IgG/lambda-expressing B cells), Raji (cytoplasmic IgG/kappa-expressing B cells), and Jurkat (T cells) cell lines. Fixed and permeabilized cells were stained with a mouse anti-human IgM monoclonal antibody (Product ## MA5-14729, red histogram) or mouse IgG1 isotype control (black histogram), each at a concentration of 1ug/ml. After incubation of the primary antibody on ice for 1 hour, the cells were stained with a FITC-conjugated goat anti-mouse IgG Fc secondary antibody (Product ## 31630) at a dilution of 1:500 for 30 minutes on ice. The representative 10,000 cells were obtained for each sample.

Flow cytometry analysis of cytoplasmic IgM expression was performed on Ramos (surface and cytoplasmic IgG/lambda-expressing B cells), Raji (cytoplasmic IgG/kappa-expressing B cells), and Jurkat (T cells) cell lines. Fixed and permeabilized cells were stained with a mouse anti-human IgM monoclonal antibody (Product ## MA5-14729, red histogram) or mouse IgG1 isotype control (black histogram), each at a concentration of 1ug/ml. After incubation of the primary antibody on ice for 1 hour, the cells were stained with a FITC-conjugated goat anti-mouse IgG Fc secondary antibody (Product ## 31630) at a dilution of 1:500 for 30 minutes on ice. The representative 10,000 cells were obtained for each sample.

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