PEBP1 Antibody (PA3-211) in WB

Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of HeLa (Lane 1), Hep G2 (Lane 2), SK-OV-3 (Lane 3), COS-7 (Lane 4), NIH/3T3 (Lane 5), C2C12 (Lane 6), PC-3 (Lane 7), and LNCaP (Lane 8). The blots were probed with Rabbit Anti-PEBP1 Polyclonal Antibody (Product ## PA3211, 1:250 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product ## A27036, 0.25 ug/ml, 1:4000 dilution). A 21 kDa band corresponding to PEBP1 was observed across the cell lines tested. The 42 kDa bands observed could potentially be PEBP1 dimers, caused by the PKC-mediated phosphorylation of PEBP1, as reported in literature. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product ## NP0321BOX), XCell SureLock™ Electrophoresis System (Product ## EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product ## LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product ## IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product ## WP20005).

Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of HeLa (Lane 1), Hep G2 (Lane 2), SK-OV-3 (Lane 3), COS-7 (Lane 4), NIH/3T3 (Lane 5), C2C12 (Lane 6), PC-3 (Lane 7), and LNCaP (Lane 8). The blots were probed with Rabbit Anti-PEBP1 Polyclonal Antibody (Product ## PA3211, 1:250 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product ## A27036, 0.25 ug/ml, 1:4000 dilution). A 21 kDa band corresponding to PEBP1 was observed across the cell lines tested. The 42 kDa bands observed could potentially be PEBP1 dimers, caused by the PKC-mediated phosphorylation of PEBP1, as reported in literature. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product ## NP0321BOX), XCell SureLock™ Electrophoresis System (Product ## EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product ## LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product ## IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product ## WP20005).

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