ERK1/ERK2 Antibody (MA5-15605) in Flow

Flow cytometry analysis of p42,44 ERK (1,2) was done on Jurkat cells. Cells were fixed, permeabilized and stained with a p42,44 ERK (1,2) mouse monoclonal antibody (Product ## MA5-15605, blue histogram) at a dilution of 1:100. After incubation of the primary antibody on ice for an hour, the cells were stained with a Goat anti-Mouse IgG (H+L) Secondary Antibody, DyLight 680 conjugate (Product ## 35519) at a dilution of 1:50 for at least 30 minutes on ice. A representative 10,000 cells were acquired for each sample. The black histogram represents unstained control cells and the red histogram represents no-primary-antibody control.

Flow cytometry analysis of p42,44 ERK (1,2) was done on Jurkat cells. Cells were fixed, permeabilized and stained with a p42,44 ERK (1,2) mouse monoclonal antibody (Product ## MA5-15605, blue histogram) at a dilution of 1:100. After incubation of the primary antibody on ice for an hour, the cells were stained with a Goat anti-Mouse IgG (H+L) Secondary Antibody, DyLight 680 conjugate (Product ## 35519) at a dilution of 1:50 for at least 30 minutes on ice. A representative 10,000 cells were acquired for each sample. The black histogram represents unstained control cells and the red histogram represents no-primary-antibody control.

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