Principle of BacMam Reagents

BacMam 2.0 GFP particles enter the cell by endocytosis. After endosomal escape the BacMam DNA moves to the nucleus where only the transgene is expressed; baculovirus genes are not recognized in mammalian cells—hence no virus replication and no toxicity. Transgene expression begins 4-6 hours after transduction. Peak gene expression is generally reached after 24 to 48 h and lasts from 4 to 5 days in transformed cells to more than 2 weeks in slowly dividing cell types.

BacMam 2.0 GFP particles enter the cell by endocytosis. After endosomal escape the BacMam DNA moves to the nucleus where only the transgene is expressed; baculovirus genes are not recognized in mammalian cells—hence no virus replication and no toxicity. Transgene expression begins 4-6 hours after transduction. Peak gene expression is generally reached after 24 to 48 h and lasts from 4 to 5 days in transformed cells to more than 2 weeks in slowly dividing cell types.

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