Three proteins detected simultaniously on one blot
Simultaneous detection of three proteins on a single blot using Qdot® secondary antibody conjugates. A western blot containing serial dilutions (20–3 µg protein) of lysates from unstimulated (lanes 2–5) and hEGF-stimulated (lanes 6–9) A431 cells was probed with mouse anti-EGFR, rabbit anti–phospho-EGFR, and chicken anti-GAPDH antibodies, followed by (A) WesternDot® 800 goat anti-mouse (pseudocolored blue), (B) WesternDot® 605 goat anti-rabbit (pseudocolored red), and (C) WesternDot® 655 goat anti-chicken (pseudocolored green) conjugates. (D) The merged image shows overlaid red and blue bands as purple. The blot contains MagicMark™ XP Western Protein Standard (lane 1, Cat. No. LC5603) and was imaged using the Fujifilm® LAS-4000 gel imager.