Fluorescence signal from Pacific Blue™ Click-iT® Plus EdU Flow Cytometry Assay Kit and Mouse Anti-Human mAb PE Conjugate.
Jurkat (human T-cell leukemia) cells were treated with 10 µM EdU for 2 hours, stained with CD3 mouse anti-human mAb PE conjugate (Cat. No MHCD0304) and detected according to the recommended staining protocol. The figures show a clear separation of proliferating cells that have incorporated EdU and nonproliferating cells that have not. Panel A shows data from cells labeled with Pacific Blue™ picolyl azide analyzed on an Attune® Acoustic Focusing Cytometer using 405 nm excitation and a 450/40 nm bandpass emission filter; Panel B shows the same cells using 488 nm excitation and a 575/24 nm bandpass emission filter. The black outlined histogram is the cells stained with mouse anti-human CD3 mAb PE and Click-iT® Plus EdU Pacific Blue™ picolyl azide. The gray outlined histogram is the Hu CD3 PE positive control cells treated the same but without copper in the reaction.