CRISPR/Cas9-mediated cleavage efficiency
Gel image of a cleavage assay using the GeneArt® Genomic Cleavage Detection Assay (Cat. No. A24372) for the HPRT locus. (A) Results using the GeneArt® CRISPR Nuclease OFP Vector expressing HPRT-specific CRISPR RNA. (B) Results obtained using the GeneArt® CRISPR Nuclease CD4 Vector expressing HPRT-specific CRISPR RNA. Following transfection into HeLa cells, triplicate cleavage assays were performed and the percentage of indels were calculated.