Sandwich ELISA analysis of ABfinity™ EGF Recombinant Rabbit Monoclonal Antibody
Sandwich ELISA analysis was performed using ABfinity™ EGF Recombinant Rabbit Monoclonal Antibody at 2 µg/mL. A ten point standard curve was plotted with full length active recombinant EGF protein as a standard with concentrations ranging from 0.3 pg/mL to 12.5 ng/mL. An anti-EGF antibody conjugated to biotin was used as a detector at a concentration of 2 µg/mL. A non-linear regression analysis (4 PL) was performed and LOD and LOQ for the antibody pair was determined.