Reducing endogenous background with the Endogenous Biotin–Blocking Kit

A549 cells were fixed and permeabilized, treated (A) or not treated (B) with the Endogenous Biotin–Blocking Kit, labeled with a primary antibody against mouse golgin-97 followed by DSB-X biotin–conjugated goat anti–mouse IgG, then stained with Alexa Fluor® 488 streptavidin (green), Alexa Fluor® 647 phalloidin (magenta), and NucBlue® Fixed Cell ReadyProbes® Reagent (blue). Note the green fluorescence produced by the labeled streptavidin is punctate and limited to the target Golgi apparatus in the treated cell (A), but there is considerable diffuse, nonspecific green fluorescence in the untreated cell (B), in addition to the punctate staining. The image was acquired at 60x magnification with a Nikon® E800 upright microscope.

A549 cells were fixed and permeabilized, treated (A) or not treated (B) with the Endogenous Biotin–Blocking Kit, labeled with a primary antibody against mouse golgin-97 followed by DSB-X biotin–conjugated goat anti–mouse IgG, then stained with Alexa Fluor® 488 streptavidin (green), Alexa Fluor® 647 phalloidin (magenta), and NucBlue® Fixed Cell ReadyProbes® Reagent (blue). Note the green fluorescence produced by the labeled streptavidin is punctate and limited to the target Golgi apparatus in the treated cell (A), but there is considerable diffuse, nonspecific green fluorescence in the untreated cell (B), in addition to the punctate staining. The image was acquired at 60x magnification with a Nikon® E800 upright microscope.

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