Greater enrichment of synaptic proteins in samples prepared using the Syn-PER Reagent compared to homebrew reagent

Total protein (10µg) from mouse brain tissue homogenates (H), cytosol (C) fraction, and synaptosome suspension (Syn) were analyzed by Western blot. The total protein yield in the synaptosome suspension produced from fresh mouse brain (200mg) was three-fold greater using the Syn-PER Reagent compared to a typical homebrew reagent.

Total protein (10µg) from mouse brain tissue homogenates (H), cytosol (C) fraction, and synaptosome suspension (Syn) were analyzed by Western blot. The total protein yield in the synaptosome suspension produced from fresh mouse brain (200mg) was three-fold greater using the Syn-PER Reagent compared to a typical homebrew reagent.

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