Protein identification of rat mitochondrial extract separated by 2D electrophoresis and stained with three different gel stains

Extract was electrophoresed in three identical gels (see figure above) and stained with Thermo Scientific Pierce Silver Stain for Mass Spectrometry, an MS-compatible stain from Supplier X and and Thermo Scientific GelCode Blue Stain Reagent (Part No. 24590). Ten spots were identified that stained well under all staining conditions. Spots were excised and the gel pieces were reduced, alkylated and typsinized using the Thermo Scientific Pierce In-Gel Tryptic Digestion Kit (Part No. 89871). Finally, MALDI-Ion Trap MS was performed on an Agilent Technologies LC/MSD Trap XCT. Peptide mass fingerprinting was performed with ProteinProspector. All proteins identified by peptide fragment mapping are known mitochondrial proteins.

Extract was electrophoresed in three identical gels (see figure above) and stained with Thermo Scientific Pierce Silver Stain for Mass Spectrometry, an MS-compatible stain from Supplier X and and Thermo Scientific GelCode Blue Stain Reagent (Part No. 24590). Ten spots were identified that stained well under all staining conditions. Spots were excised and the gel pieces were reduced, alkylated and typsinized using the Thermo Scientific Pierce In-Gel Tryptic Digestion Kit (Part No. 89871). Finally, MALDI-Ion Trap MS was performed on an Agilent Technologies LC/MSD Trap XCT. Peptide mass fingerprinting was performed with ProteinProspector. All proteins identified by peptide fragment mapping are known mitochondrial proteins.

Related Products

Related Images

CD335 (NKp46) Antibody (63335182) in RE Go ›

CD223 (LAG-3) Antibody (56223942) in TM Go ›