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SuperSignal Western Blot Enhancer is compatible with chromogenic and fluorescent detection methods
HeLa cell lysate was separated by electrophoresis, transferred to nitrocellulose (Top) or Low-Fluorescence PVDF (Part No. 22860) (Bottom) and Western blotting was performed using the conventional method (Left) or using the SuperSignal Western Blot Enhancer protocol (Right). For chromogenic detection (Top), the nitrocellulose membranes were blocked with SuperBlock in TBS (Part No. 37535) and probed with rabbit anti-ß-Catenin (LabVision Part No. PAb RB-1491-PABX) at 0.2µg/mL followed by alkaline phosphatase-conjugated goat anti-rabbit IgG (Part No. 31340) at 0.04µg/mL. Detection was performed with Thermo Scientific 1-Step NBT/BCIP Substrate (Part No. 34042). For fluorescent detection (Bottom) , the PVDF membranes were blocked with Blocker BLOTTO in TBS (Part No. 37530) and probed with rabbit anti-MAP Kinase at 1µg/mL followed by Thermo Scientific DyLight 488-conjugated and goat anti-rabbit IgG (Part No. 35552) at 0.1µg/mL. Membranes were imaged with the GE Healthcare Typhoon™ 9410 Instrument.
