False-color image of free Ca2+ concentration in a Purkinje neuron from embryonic mouse cerebellum.
False-color image of free Ca2+ concentration in a Purkinje neuron from embryonic mouse cerebellum. Neurons were grown in dispersed tissue culture for 12 days, loaded with the pentapotassium salt of fura-2 (Cat. no. F1200) using a microelectrode and then challenged with trans-ACPD, an agonist of metabotropic glutamate receptors, in the absence of extracellular Ca2+. The composite image, which represents the ratio of images obtained with excitation at 340 nm and 380 nm, reveals the mobilization of internal Ca2+ stores without contribution from Ca2+ influx. The image was contributed by D.J. Linden, Johns Hopkins University, and M. Smeyne and J.A. Connor, Roche Institute of Molecular Biology.
REF-52 fibroblasts. Cyclic AMP Fluorosensor (FlCRhR) and fura-2 AM Go ›
Pseudocolored images of changes in intracellular free Ca2+ in AtT-20/D16v-F2 cells, monitored at 9 sec intervals with fluo-4, AM (F14201, F14202, F14217, F23917). Go ›
Figure 5: IgG binding capacity vs. salt concentration Go ›
Figure 4: Resolution as a function of protein loading Go ›
Developing Drosophila embryo Go ›
Cytoskeleton of a mixed population of granule neurons and glial cells Go ›