Dual-emission ratiometric measurement of lysosomal pH using LysoSensor™ Yellow/Blue dextran.
Dual-emission ratiometric measurement of lysosomal pH using LysoSensor™ Yellow/Blue dextran (Cat. no. L22460). MDCK cells labeled with the fluorescent dextran were exposed to pH-calibration buffers (pH 3.5, top panel; or pH 6.0, bottom panel) in the presence of nigericin (Cat. no. N1495) and monensin. Pseudocolored images were constructed from two emission images at 450 ± 33 nm and 510 ± 20 nm, both excited at 365 ± 8 nm. (In these images, fluorescence near pH 3 has been pseudocolored blue and fluorescence near pH 6 pseudocolored yellow-green.) (Chem Biol 6, 411 (1999))
Bacteriophage T4 replication proteins stained with SYPRO® Orange Protein Gel Stain. Go ›
Madin-Darby canine kidney cells. LysoSensor Yellow/Blue DND-160. Go ›
Rhizoid cells from the marine alga Fucus serratus were pressure-microinjected with 10,000 MW Calcium Green™-1 dextran and 10,000 MW Texas Red® dextran. Go ›
Secondary motor neurons in a male western mosquitofish (Gambusia affinis affinis) spinal cord. Go ›
NPM1 Antibody (MA325200A488) in IF Go ›
MAP2 Antibody (PA5-17646) in IF Go ›