Detection of an oligohistidine fusion protein with the Penta·His mouse monoclonal antibody.
Detection of an oligohistidine fusion protein with the Penta·His mouse monoclonal antibody. Twofold serial dilutions of an Escherichia coli lysate containing overexpressed oligomycin sensitivity–conferring protein (OSCP) fused with a hexahistidine domain were run an SDS-polyacrylamide gel and blotted onto a PVDF membrane. The blot was stained with the SYPRO Ruby protein blot stain (S11791) to detect the entire protein profile (top). After imaging, the blot was incubated with Penta·His mouse IgG1 monoclonal antibody (P21315), followed by immunodetection using an alkaline phosphatase conjugate of goat anti–mouse antibody in conjunction with DDAO phosphate (bottom).
Bacteriophage T4 replication proteins stained with SYPRO® Orange Protein Gel Stain. Go ›
2D gel stained with the SYPRO Ruby protein gel stain and the Pro-Q Emerald 300 reagent. Go ›
Figure 5: IgG binding capacity vs. salt concentration Go ›
Figure 4: Resolution as a function of protein loading Go ›
Increase in accuracy and precision using the Qubit® Go ›
Performance of the Qubit® dsDNA HS Assay Go ›