Visualization of total protein and phosphoproteins in a 2D gel.
Visualization of total protein and phosphoproteins in a 2D gel. Proteins from a Jurkat T-cell lymphoma line cell lysate were separated by 2D gel electrophoresis and stained with Pro-Q Diamond phosphoprotein gel stain (P33300, P33301, P33302, blue) followed by SYPRO Ruby protein gel stain (S12000, S12001, S21900, red). After each dye staining, the gel was imaged on an FLA-3000 scanner (Fuji). The digital images were acquired using Z3 software (Compugen), and the resulting composite image was digitally pseudocolored and overlaid.