Calcium flux in porcine left atrial appendage progenitor cells transfected with Premo™ Cameleon calcium sensor.
Porcine left atrial appendage progenitor cells were transfected with Premo™ Cameleon calcium sensor (P36207, P36208); ATP (20 µM final concentration) was applied to the cells the following day and the cells were imaged using a Zeiss 5 Live high-speed confocal system (Carl Zeiss MicroImaging). Excitation was with a 405 nm diode laser (50 mw) operated at 50% power. Emission was collected simultaneously on two linear CCD detectors using a 490 nm dichroic mirror to split the beam through a 415–480 nm bandpass filter for CFP and a 550 nm longpass filter for YFP. Images were collected at a rate of 10 frames per second (512 x 512 pixels) using a 40x Plan-Neofluar 1.3 NA oil immersion objective lens.
REF-52 fibroblasts. Cyclic AMP Fluorosensor (FlCRhR) and fura-2 AM Go ›
Pseudocolored images of changes in intracellular free Ca2+ in AtT-20/D16v-F2 cells, monitored at 9 sec intervals with fluo-4, AM (F14201, F14202, F14217, F23917). Go ›
HeLa cells transduced with Organelle Lights™ Mito-GFP. Go ›
Four-color staining of a muntjac cell with probes for cytoskeletal, nuclear and mitochondrial proteins Go ›
Imaging autophagy in live HeLa cells with CellLight® reagents for mitochondria and lysosomes: Go ›
Porcine primary skeletal muscle cell transduced with Organelle Lights fluorescent proteins. Go ›
Design Flow Chart Go ›
Choosing the most suitable format Go ›